• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于小鼠大脑容积成像的多光束连续轴向扫描双光子显微镜

Multibeam continuous axial scanning two-photon microscopy for volumetric imaging in mouse brain.

作者信息

Ataka Mitsutoshi, Otomo Kohei, Enoki Ryosuke, Ishii Hirokazu, Tsutsumi Motosuke, Kozawa Yuichi, Sato Shunichi, Nemoto Tomomi

机构信息

National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki 444-8787, Japan.

Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki 444-8787, Japan.

出版信息

Biomed Opt Express. 2024 Jan 25;15(2):1089-1101. doi: 10.1364/BOE.514826. eCollection 2024 Feb 1.

DOI:10.1364/BOE.514826
PMID:38404301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10890896/
Abstract

This study presents an alternative approach for two-photon volumetric imaging that combines multibeam lateral scanning with continuous axial scanning using a confocal spinning-disk scanner and an electrically focus tunable lens. Using this proposed system, the brain of a living mouse could be imaged at a penetration depth of over 450 μm from the surface. volumetric Ca imaging at a volume rate of 1.5 Hz within a depth range of 130-200 μm, was segmented with an axial pitch of approximately 5-µm and revealed spontaneous activity of neurons with their 3D positions. This study offers a practical microscope design equipped with compact scanners, a simple control system, and readily adjustable imaging parameters, which is crucial for the widespread adoption of two-photon volumetric imaging.

摘要

本研究提出了一种用于双光子体积成像的替代方法，该方法将多光束横向扫描与使用共焦旋转盘扫描仪和电聚焦可调透镜的连续轴向扫描相结合。使用该提议的系统，可以对活体小鼠大脑从表面起超过450μm的穿透深度进行成像。在130 - 200μm深度范围内以1.5Hz的体积速率进行体积钙成像，轴向间距约为5μm，显示出神经元的自发活动及其三维位置。本研究提供了一种配备紧凑扫描仪、简单控制系统和易于调节成像参数的实用显微镜设计，这对于双光子体积成像的广泛应用至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/b621995e8fce/boe-15-2-1089-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/8662a75301a9/boe-15-2-1089-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/ded1fd979dd1/boe-15-2-1089-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/aa64743bf2ec/boe-15-2-1089-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/b621995e8fce/boe-15-2-1089-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/8662a75301a9/boe-15-2-1089-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/ded1fd979dd1/boe-15-2-1089-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/aa64743bf2ec/boe-15-2-1089-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0857/10890896/b621995e8fce/boe-15-2-1089-g004.jpg

相似文献

1
Multibeam continuous axial scanning two-photon microscopy for volumetric imaging in mouse brain.用于小鼠大脑容积成像的多光束连续轴向扫描双光子显微镜
Biomed Opt Express. 2024 Jan 25;15(2):1089-1101. doi: 10.1364/BOE.514826. eCollection 2024 Feb 1.
2
50 Hz volumetric functional imaging with continuously adjustable depth of focus.具有连续可调焦深的50赫兹容积功能成像。
Biomed Opt Express. 2018 Mar 28;9(4):1964-1976. doi: 10.1364/BOE.9.001964. eCollection 2018 Apr 1.
3
Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens.使用电可调透镜对神经元细胞群体进行快速双层双光子成像。
Biomed Opt Express. 2011 Jul 1;2(7):2035-46. doi: 10.1364/BOE.2.002035. Epub 2011 Jun 23.
4
Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.通过电调谐透镜在共振频率下实现线扫描共焦显微镜的快速体积成像。
Opt Express. 2022 May 23;30(11):19152-19164. doi: 10.1364/OE.450745.
5
Optical axial scanning in confocal microscopy using an electrically tunable lens.使用电调谐透镜在共聚焦显微镜中进行光轴扫描。
Biomed Opt Express. 2014 Jan 30;5(2):645-52. doi: 10.1364/BOE.5.000645. eCollection 2014 Feb 1.
6
Reflectance confocal microscopy of oral epithelial tissue using an electrically tunable lens.使用电可调透镜对口腔上皮组织进行反射式共聚焦显微镜检查。
Proc SPIE Int Soc Opt Eng. 2014;8935. doi: 10.1117/12.2037046. Epub 2014 Feb 27.
7
Volumetric HiLo microscopy employing an electrically tunable lens.采用电可调透镜的体积式高低显微镜术。
Opt Express. 2016 Jun 27;24(13):15029-41. doi: 10.1364/OE.24.015029.
8
Remote Axial Tuning in Microscopy Utilizing Hydrogel-Driven Tunable Liquid Lens.利用水凝胶驱动的可调谐液体透镜在显微镜中进行远程轴向调谐
J Microelectromech Syst. 2016 Apr;25(2):304-310. doi: 10.1109/jmems.2016.2518922. Epub 2016 Feb 3.
9
Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning.利用带有主动轴向扫描的微型光纤耦合显微镜在自由移动的小鼠中进行三维双光子脑成像。
Sci Rep. 2018 May 25;8(1):8108. doi: 10.1038/s41598-018-26326-3.
10
MEMS-BASED 3D CONFOCAL SCANNING MICROENDOSCOPE USING MEMS SCANNERS FOR BOTH LATERAL AND AXIAL SCAN.基于微机电系统（MEMS）的三维共聚焦扫描微型内窥镜，采用MEMS扫描器进行横向和轴向扫描。
Sens Actuators A Phys. 2014 Aug 15;215:89-95. doi: 10.1016/j.sna.2013.09.035.

本文引用的文献

1
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations.高速低光活体双光子电压成像大型神经元群体。
Nat Methods. 2023 Jul;20(7):1095-1103. doi: 10.1038/s41592-023-01820-3. Epub 2023 Mar 27.
2
Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit.实时去噪使得高灵敏度荧光延时成像能够突破散粒噪声极限。
Nat Biotechnol. 2023 Feb;41(2):282-292. doi: 10.1038/s41587-022-01450-8. Epub 2022 Sep 26.
3
Single-scan volumetric imaging throughout thick tissue specimens by one-touch installable light-needle creating device.
一键式可安装光针创建设备实现厚组织标本的单次扫描容积成像。
Sci Rep. 2022 Jun 21;12(1):10468. doi: 10.1038/s41598-022-14647-3.
4
Wavefront engineered light needle microscopy for axially resolved rapid volumetric imaging.用于轴向分辨快速体积成像的波前工程光针显微镜。
Biomed Opt Express. 2022 Feb 24;13(3):1702-1717. doi: 10.1364/BOE.449329. eCollection 2022 Mar 1.
5
High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy.使用光珠显微镜以细胞分辨率进行高速、皮质全容积神经活动记录。
Nat Methods. 2021 Sep;18(9):1103-1111. doi: 10.1038/s41592-021-01239-8. Epub 2021 Aug 30.
6
Reinforcing neuron extraction and spike inference in calcium imaging using deep self-supervised denoising.利用深度自监督去噪增强钙成像中的神经元提取和尖峰推断。
Nat Methods. 2021 Nov;18(11):1395-1400. doi: 10.1038/s41592-021-01225-0. Epub 2021 Aug 16.
7
Electrically Tunable Lenses: A Review.电可调透镜：综述
Front Robot AI. 2021 Jun 9;8:678046. doi: 10.3389/frobt.2021.678046. eCollection 2021.
8
Synthesis of Conserved Odor Object Representations in a Random, Divergent-Convergent Network.在一个随机发散-收敛网络中合成保守气味物体表示。
Neuron. 2020 Oct 28;108(2):367-381.e5. doi: 10.1016/j.neuron.2020.07.029. Epub 2020 Aug 18.
9
High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores.基于高峰值功率 918nm 激光光源的双光子旋转盘显微镜用于检测绿色荧光染料。
Biochem Biophys Res Commun. 2020 Aug 20;529(2):238-242. doi: 10.1016/j.bbrc.2020.05.213. Epub 2020 Jun 22.
10
Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator.通过靶向细胞体的钙指示剂对密集神经群体进行精确钙成像。
Neuron. 2020 Aug 5;107(3):470-486.e11. doi: 10.1016/j.neuron.2020.05.029. Epub 2020 Jun 26.