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编码牛精核糖核酸酶的克隆cDNA的序列分析

Sequence analysis of a cloned cDNA coding for bovine seminal ribonuclease.

作者信息

Palmieri M, Carsana A, Furia A, Libonati M

出版信息

Eur J Biochem. 1985 Oct 15;152(2):275-7. doi: 10.1111/j.1432-1033.1985.tb09194.x.

DOI:10.1111/j.1432-1033.1985.tb09194.x
PMID:3840434
Abstract

The sequence of a cloned cDNA coding for bovine seminal ribonuclease, an enzyme secreted in the bull seminal vesicles, was determined. The cDNA starts at the amino acid residue 47 and terminates 12 nucleotides beyond the consensus sequence AAUAAA in the 3' non-coding region of the mRNA. Northern blotting analysis shows that the mRNA for bovine seminal ribonuclease consists of about 950 nucleotides, a value that is similar to that of other mRNAs coding for ribonucleases of the pancreatic type.

摘要

测定了编码牛精囊核糖核酸酶(一种在公牛精囊中分泌的酶)的克隆cDNA序列。该cDNA从氨基酸残基47处开始,在mRNA的3'非编码区中位于共有序列AAUAAA下游12个核苷酸处终止。Northern印迹分析表明,牛精囊核糖核酸酶的mRNA由约950个核苷酸组成,这一数值与其他编码胰腺型核糖核酸酶的mRNA相似。

相似文献

1
Sequence analysis of a cloned cDNA coding for bovine seminal ribonuclease.编码牛精核糖核酸酶的克隆cDNA的序列分析
Eur J Biochem. 1985 Oct 15;152(2):275-7. doi: 10.1111/j.1432-1033.1985.tb09194.x.
2
The differential pattern of tissue-specific expression of ruminant pancreatic type ribonucleases may help to understand the evolutionary history of their genes.反刍动物胰腺型核糖核酸酶组织特异性表达的差异模式可能有助于了解其基因的进化史。
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Mol Biol Evol. 1990 Jan;7(1):29-44. doi: 10.1093/oxfordjournals.molbev.a040585.
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Bovine seminal ribonuclease precursor synthesized in vitro.体外合成的牛精浆核糖核酸酶前体。
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Cloning of cDNA encoding the complete precursor for bovine seminal ribonuclease.编码牛精核糖核酸酶完整前体的cDNA的克隆
Nucleic Acids Res. 1990 Feb 25;18(4):1057. doi: 10.1093/nar/18.4.1057.
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Synthesis of seminal ribonuclease in isolated lobules of bull seminal vesicles.
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Structure of the bovine pancreatic ribonuclease gene: the unique intervening sequence in the 5' untranslated region contains a promoter-like element.牛胰核糖核酸酶基因的结构:5'非翻译区中的独特间隔序列包含一个启动子样元件。
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[Ribonuclease of bull seminal vesicles (SV-1 RNase)].
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Gene expression in bovine seminal vesicles.
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引用本文的文献

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Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family.四个新型小鼠核糖核酸酶基因的分子克隆:核糖核酸酶A基因家族内的异常扩增
Nucleic Acids Res. 1997 Nov 1;25(21):4235-9. doi: 10.1093/nar/25.21.4235.
2
Molecular evolution of genes encoding ribonucleases in ruminant species.反刍动物物种中核糖核酸酶编码基因的分子进化
J Mol Evol. 1995 Dec;41(6):850-8. doi: 10.1007/BF00173164.
3
Sequences related to the ox pancreatic ribonuclease coding region in the genomic DNA of mammalian species.
哺乳动物基因组DNA中与牛胰核糖核酸酶编码区相关的序列。
J Mol Evol. 1993 Jul;37(1):29-35. doi: 10.1007/BF00170459.
4
Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues.人核糖核酸酶4(RNase 4):编码序列、染色体定位以及人类体细胞组织中两种不同转录本的鉴定
Nucleic Acids Res. 1995 Nov 11;23(21):4290-5. doi: 10.1093/nar/23.21.4290.
5
Differences in glycosylation pattern of human secretory ribonucleases.人分泌型核糖核酸酶糖基化模式的差异。
Biochem J. 1988 Oct 15;255(2):501-5.
6
Structure of the bovine pancreatic ribonuclease gene: the unique intervening sequence in the 5' untranslated region contains a promoter-like element.牛胰核糖核酸酶基因的结构:5'非翻译区中的独特间隔序列包含一个启动子样元件。
Nucleic Acids Res. 1988 Jun 24;16(12):5491-502. doi: 10.1093/nar/16.12.5491.
7
Cloning of cDNA encoding the complete precursor for bovine seminal ribonuclease.编码牛精核糖核酸酶完整前体的cDNA的克隆
Nucleic Acids Res. 1990 Feb 25;18(4):1057. doi: 10.1093/nar/18.4.1057.
8
Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland.小鼠核糖核酸酶基因Rib-1的分离:胰腺和腮腺中的结构及组织特异性表达
Nucleic Acids Res. 1991 Dec 25;19(24):6935-41. doi: 10.1093/nar/19.24.6935.
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Molecular cloning of the gene encoding the bovine brain ribonuclease and its expression in different regions of the brain.编码牛脑核糖核酸酶的基因的分子克隆及其在脑不同区域的表达。
Nucleic Acids Res. 1991 Dec 11;19(23):6469-74. doi: 10.1093/nar/19.23.6469.