编码牛精核糖核酸酶的克隆cDNA的序列分析
Sequence analysis of a cloned cDNA coding for bovine seminal ribonuclease.
作者信息
Palmieri M, Carsana A, Furia A, Libonati M
出版信息
Eur J Biochem. 1985 Oct 15;152(2):275-7. doi: 10.1111/j.1432-1033.1985.tb09194.x.
The sequence of a cloned cDNA coding for bovine seminal ribonuclease, an enzyme secreted in the bull seminal vesicles, was determined. The cDNA starts at the amino acid residue 47 and terminates 12 nucleotides beyond the consensus sequence AAUAAA in the 3' non-coding region of the mRNA. Northern blotting analysis shows that the mRNA for bovine seminal ribonuclease consists of about 950 nucleotides, a value that is similar to that of other mRNAs coding for ribonucleases of the pancreatic type.
测定了编码牛精囊核糖核酸酶（一种在公牛精囊中分泌的酶）的克隆cDNA序列。该cDNA从氨基酸残基47处开始，在mRNA的3'非编码区中位于共有序列AAUAAA下游12个核苷酸处终止。Northern印迹分析表明，牛精囊核糖核酸酶的mRNA由约950个核苷酸组成，这一数值与其他编码胰腺型核糖核酸酶的mRNA相似。
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