Elghobashy Roufaida M, Rashed Shimaa A, Fakhry Amal M, Mostafa Reham M, Essawy Heba S, El-Darier Salama M
Biology and Geology Department, Faculty of Education, Alexandria University, Alexandria, Egypt.
Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Heliyon. 2024 Feb 18;10(4):e26381. doi: 10.1016/j.heliyon.2024.e26381. eCollection 2024 Feb 29.
Biological control of undesirable weeds associated with crop cultivation is a sustainable approach that can reduce chemical herbicide dependence. The current study aimed to assess the bio-herbicidal potential of the donor species Vahl. on germination efficiency as well as various growth and physiological parameters of the recipient species L., a major broad bean pest ( L.). To assess the greatest inhibitory allelopathic effect on the recipient species in mixed ( L and L.) and pure cultures (each one separately), two experiments were conducted under laboratory conditions. A Petri dish experiment using shoot aqueous extract (5%, 10%, 20%, and 40%) and a pot experiment using shoot crude powder (1%, 2%, 5%, and 10%) were conducted to investigate its biological activity on some growth and physiological parameters of both crop and weed species. underwent a general phytochemical screening that revealed a high production of allelochemicals, which are secondary metabolites and may have a function like that of natural herbicides. The result showed that the germination of seeds in both pure and mixed cultures was not significantly affected by low levels of shoot aqueous extract treatments in pure and mixed cultures, in contrast, those recorded for gradually dropped as levels of O. vaginalis increased in both cultures. Results recorded a significant increase in total phenolics of shoots and roots under different treatments, except at the high concentrations of crude powder at the donor species level (5 and10%). A reduction in the total phenolic and flavonoid fractions was observed in roots under varying concentration treatments. Conversely, under high concentration treatments, flavonoids decreased in the roots of the mixed culture of but increased in the shoots. In conclusion, allelopathy can be used to suppress weeds in field crops. The study confirmed the use of into current weed control techniques. could be explored further for weed suppression in the field.
对与作物种植相关的有害杂草进行生物防治是一种可持续的方法，可减少对化学除草剂的依赖。当前的研究旨在评估供体物种Vahl.对受体物种L.（一种主要的蚕豆害虫）的发芽效率以及各种生长和生理参数的生物除草潜力。为了评估在混合培养（L.和L.）和纯培养（每种分别培养）中对受体物种的最大抑制化感作用，在实验室条件下进行了两项实验。进行了一项使用Vahl.地上部分水提取物（5%、10%、20%和40%）的培养皿实验以及一项使用Vahl.地上部分粗粉（1%、2%、5%和10%）的盆栽实验，以研究其对作物和杂草物种某些生长和生理参数的生物活性。对Vahl.进行了一般植物化学筛选，结果显示其大量产生化感物质，这些化感物质是次生代谢产物，可能具有天然除草剂的功能。结果表明，在纯培养和混合培养中，低水平的Vahl.地上部分水提取物处理对L.种子的发芽没有显著影响，相反，随着两种培养中Vahl.水平的增加，L.的发芽率逐渐下降。结果表明，除了供体物种水平的高浓度粗粉（5%和10%）外，不同处理下L.地上部分和根部的总酚含量显著增加。在不同浓度处理下，L.根部的总酚和黄酮类成分减少。相反，在高浓度处理下，混合培养的L.根部黄酮类物质减少，但地上部分增加。总之，化感作用可用于抑制大田作物中的杂草。该研究证实了将Vahl.应用于当前的杂草控制技术中。Vahl.在田间杂草抑制方面还有进一步探索的潜力。
