Institute of Plant Pathology, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
Nat Prod Res. 2011 Apr;25(7):730-40. doi: 10.1080/14786419.2010.528757.
The herbicidal potential of culture filtrates of four Trichoderma spp., namely Trichoderma harzianum Rifai, Trichoderma pseudokoningii Rifai, Trichoderma reesei Simmons and Trichoderma viride Pers., was evaluated against two problematic weeds of wheat, Phalaris minor L. and Rumex dentatus L. In laboratory bioassays, generally, metabolites of all four Trichoderma species significantly reduced various root and shoot growth parameters of the two target weed species. The original concentrations of the culture filtrates of all Trichoderma spp., except T. harzianum, significantly reduced various parameters of root and shoot growth of wheat seedlings. In a foliar spray bioassay, the culture filtrates of all four Trichoderma spp. significantly diminished root and shoot biomass of R. dentatus. The effect of these filtrates on the shoot growth of P. minor and wheat was not significant. Culture filtrates of the four Trichoderma species were successively extracted with butanol, n-hexane, chloroform and ethyl acetate. In detached leaf injection bioassays, n-hexane fractions (3 mg mL(-1)) of T. pseudokoningii, T. reesei and T. viride, and ethyl acetate fractions of T. horzianum and T. pseudokoningii were found to be toxic against R. dentatus. This study concludes that the culture filtrates of Trichoderma species have herbicidal potential in the control of R. dentatus.
四种木霉（哈茨木霉、拟康氏木霉、里氏木霉和绿色木霉）的培养液对小麦两种难除杂草（雀麦和酸模叶蓼）的除草潜力进行了评估。在实验室生物测定中，四种木霉的代谢物通常显著降低了两种靶标杂草的各种根和茎生长参数。除哈茨木霉外，所有木霉培养液的原始浓度都显著降低了小麦幼苗的各种根和茎生长参数。在叶面喷雾生物测定中，四种木霉的培养液都显著降低了酸模叶蓼的根和茎生物量。这些滤液对雀麦和小麦的茎生长影响不显著。用正丁醇、正己烷、氯仿和乙酸乙酯依次对四种木霉的培养液进行了萃取。在离体叶片注射生物测定中，发现拟康氏木霉、里氏木霉和绿色木霉的正己烷级分（3mg/mL）以及哈茨木霉和拟康氏木霉的乙酸乙酯级分对酸模叶蓼有毒。本研究得出结论，木霉培养液具有控制酸模叶蓼的除草潜力。
