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四周来每日甜菊糖苷饮料与蔗糖饮料对健康成年人肠道微生物组的比较。

Comparison of a Daily Steviol Glycoside Beverage compared with a Sucrose Beverage for Four Weeks on Gut Microbiome in Healthy Adults.

机构信息

Frontage Laboratories (BRI), Vancouver, British Columbia, Canada.

Cargill R&D Center, Plymouth, Minnesota, USA.

出版信息

J Nutr. 2024 Apr;154(4):1298-1308. doi: 10.1016/j.tjnut.2024.01.032. Epub 2024 Feb 24.

DOI:10.1016/j.tjnut.2024.01.032

PMID:38408729

Abstract

BACKGROUND

Recent studies suggest that some nonnutritive sweeteners (NNS) have deleterious effects on the human gut microbiome (HGM). The effect of steviol glycosides on the HGM has not been well studied.

OBJECTIVE

We aimed to evaluate the effects of stevia- compared with sucrose-sweetened beverages on the HGM and fecal short-chain fatty acid (SCFA) profiles.

METHODS

Using a randomized, double-blinded, parallel-design study, n = 59 healthy adults [female/male, n = 36/23, aged 31±9 y, body mass index (BMI): 22.6±1.7 kg/m] consumed 16 oz of a beverage containing either 25% of the acceptable daily intake (ADI) of stevia or 30 g of sucrose daily for 4 weeks followed by a 4-week washout. At weeks 0 (baseline), 4, and 8, the HGM was characterized via shotgun sequencing, fecal SCFA concentrations were measured using ultra-high performance liquid chromatography-tandem mass spectrometry and anthropometric measurements, fasting serum glucose, insulin and lipids, blood pressure, pulse, and 3-d diet records were obtained.

RESULTS

There were no significant differences in the HGM or fecal SCFA between the stevia and sucrose groups at baseline (P > 0.05). At week 4 (after intervention), there were no significant differences in the HGM at the phylum, family, genus, or species level between the stevia and sucrose groups and no significant differences in fecal SCFA. At week 4, BMI had increased by 0.3 kg/m (P = 0.013) in sucrose compared with stevia, but all other anthropometric and cardiometabolic measures and food intake did not differ significantly (P > 0.05). At week 8 (after washout), there were no significant differences in the HGM, fecal SFCA, or any anthropometric or cardiometabolic measure between the stevia and sucrose groups (P > 0.05).

CONCLUSIONS

Daily consumption of a beverage sweetened with 25% of the ADI of stevia for 4 weeks had no significant effects on the HGM, fecal SCFA, or fasting cardiometabolic measures, compared with daily consumption of a beverage sweetened with 30 g of sucrose.

TRIAL REGISTRATION

clinicaltrials.gov as NCT05264636.

摘要

背景

最近的研究表明，一些非营养性甜味剂（NNS）对人体肠道微生物组（HGM）有有害影响。甜菊糖苷对 HGM 的影响尚未得到很好的研究。

目的

我们旨在评估甜菊糖与蔗糖甜味饮料对 HGM 和粪便短链脂肪酸（SCFA）谱的影响。

方法

采用随机、双盲、平行设计研究，共纳入 59 名健康成年人[女性/男性，n=36/23，年龄 31±9 岁，体重指数（BMI）：22.6±1.7 kg/m]，分别饮用含 25%可接受日摄入量（ADI）甜菊糖或 30 g 蔗糖的饮料 16 盎司，持续 4 周，然后进行 4 周洗脱期。在第 0 周（基线）、4 周和 8 周时，通过高通量测序对 HGM 进行特征分析，使用超高效液相色谱-串联质谱法测量粪便 SCFA 浓度，并进行人体测量、空腹血糖、胰岛素和血脂、血压、脉搏和 3 天饮食记录。

结果

基线时，甜菊糖组和蔗糖组的 HGM 或粪便 SCFA 无显著差异（P>0.05）。第 4 周（干预后）时，甜菊糖组和蔗糖组在门、科、属或种水平上的 HGM 无显著差异，粪便 SCFA 也无显著差异。第 4 周时，与甜菊糖相比，蔗糖组 BMI 增加了 0.3 kg/m（P=0.013），但其他人体测量和心血管代谢指标以及食物摄入量无显著差异（P>0.05）。第 8 周（洗脱后）时，甜菊糖组和蔗糖组的 HGM、粪便 SCFA 或任何人体测量和心血管代谢指标均无显著差异（P>0.05）。