Wietgrefe S, Zupancic M, Haase A, Chesebro B, Race R, Frey W, Rustan T, Friedman R L
Science. 1985 Dec 6;230(4730):1177-9. doi: 10.1126/science.3840915.
A complementary DNA library was constructed from messenger RNA's extracted from the brains of mice infected with the scrapie agent. The library was differentially screened with the objectives of finding clones that might be used as markers of infection and finding clones of genes whose increased expression might be correlated with the pathological changes common to scrapie and Alzheimer's disease. A gene was identified whose expression is increased in scrapie. The complementary DNA corresponding to this gene hybridized preferentially and focally to cells in the brains of scrapie-infected animals. The cloned DNA also hybridized to the neuritic plaques found with increased frequency in brains of patients with Alzheimer's disease.
从感染瘙痒病病原体的小鼠大脑中提取信使核糖核酸构建了互补脱氧核糖核酸文库。对该文库进行差异筛选，目的是找到可作为感染标志物的克隆，以及找到其表达增加可能与瘙痒病和阿尔茨海默病共有的病理变化相关的基因克隆。鉴定出一个在瘙痒病中表达增加的基因。对应于该基因的互补脱氧核糖核酸优先且局部地与瘙痒病感染动物大脑中的细胞杂交。克隆的脱氧核糖核酸也与阿尔茨海默病患者大脑中发现频率增加的神经炎性斑块杂交。
