Department of Plastic and Reconstructive Surgery, The Second Affiliated Hospital of Anhui Medical University, 678, FuRong Road, Hefei, 230601, Anhui, China.
Department of Breast Surgery, The Second Affiliated Hospital of Anhui Medical University, 678, FuRong Road, Hefei, 230601, Anhui, China.
Aesthetic Plast Surg. 2024 May;48(9):1817-1824. doi: 10.1007/s00266-024-03888-4. Epub 2024 Feb 26.
In plastic surgery, autologous fat grafts (AFG) play an important role because of their abundant supply, biocompatibility, and low rejection rate. However, the lower retention rate of fat grafts limits their widespread use. Brown adipose tissue (BAT) can promote angiogenesis and regulate the level of associated inflammation. This study explored whether BAT has a facilitative effect on fat graft retention.
We obtained white adipose tissue (WAT) from c57 mice and combined it with either BAT from c57 mice or phosphate-buffered saline (PBS) as a control. These mixtures were injected subcutaneously into the back of thymus-free nude mice. After 12 weeks, fat grafts were harvested, weighed, and analyzed.
We found that the BAT-grafted group had higher mass retention, more mature adipocytes, and higher vascularity than the other group. Further analysis revealed that BAT inhibited M1 macrophages; down-regulated IL-6, IL-1β, and TNF-β; upregulated M2 macrophages and Vascular endothelial growth factor-A (VEGFA); and promoted adipocyte regeneration by inhibiting the Wnt/β-catenin pathway, which together promoted adipose graft retention.
The study demonstrated that BAT improved adipose graft retention by promoting angiogenesis, inhibiting tissue inflammation levels and the Wnt/β-catenin pathway.
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在整形外科学中，自体脂肪移植（AFG）因其来源丰富、生物相容性好、排斥反应率低而发挥着重要作用。然而，脂肪移植的留存率较低限制了其广泛应用。棕色脂肪组织（BAT）可以促进血管生成并调节相关炎症水平。本研究探讨了 BAT 是否对脂肪移植的留存具有促进作用。
我们从 c57 小鼠中获取白色脂肪组织（WAT），并将其与来自 c57 小鼠的 BAT 或磷酸盐缓冲盐水（PBS）组合作为对照。将这些混合物注射到无胸腺裸鼠的背部皮下。12 周后，收获、称重并分析脂肪移植物。
我们发现 BAT 移植组的质量留存率更高，脂肪细胞更成熟，血管生成更多。进一步分析表明，BAT 抑制 M1 巨噬细胞；下调 IL-6、IL-1β 和 TNF-β；上调 M2 巨噬细胞和血管内皮生长因子 A（VEGFA）；通过抑制 Wnt/β-catenin 通路促进脂肪细胞再生，从而共同促进脂肪移植物的留存。
该研究表明，BAT 通过促进血管生成、抑制组织炎症水平和 Wnt/β-catenin 通路来改善脂肪移植物的留存。
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