Department of Body Contouring and Fat Grafting, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 33 Badachu Road, Shijingshan District, Beijing, 100144, People's Republic of China.
Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
Aesthetic Plast Surg. 2022 Apr;46(2):923-936. doi: 10.1007/s00266-022-02794-x. Epub 2022 Feb 15.
The optimal fat processing technique of fat grafting has not been determined. We have proved the importance of washing lipoaspirate to remove blood, but the necessity of washing when there is no obvious bleeding during liposuction is not clear.
The purpose of this study is to further investigate the effect of washing on fat graft survival and the underlying mechanisms, from the perspective of inflammation, oxidative stress and apoptosis.
To exclude the influence of blood, de-erythrocyte infranatant (dEI) isolated from lipoaspirate was obtained. Purified fat processed by cotton pad filtration mixed with dEIs after sedimentation (sedimentation group), washing (washing group) or phosphate buffer solution (control group) was transplanted to nude mice subcutaneously. Samples were harvested at 1 day and 1, 3, 8 weeks after transplantation. Volume and weight retention, histologic examination, immunostaining of perilipin-1, CD31, CD45 and Ly6g, mRNA expression of PPAR-γ, C/EBPα, VEGF, bFGF, IL-6, IL10, TNF-α, TGF-β, Bax and Bcl-2, and protein contents of 8-iso-PGF2α, IL-6, IL10, TNF-α and TGF-β were all compared among groups.
After transplantation, volume and weight retention, histologic scores, viable adipocytes and vascularization were all improved in the washing group, with increased expression of adipogenic and angiogenic genes. Compared with the sedimentation group, the washing group had milder inflammation, lower levels of oxidative stress and apoptosis.
Washing lipoaspirate to eliminate mixed components can improve fat graft survival and promote adipogenesis and angiogenesis, possibly by relieving inflammation, reducing oxidative stress injury and inhibiting apoptosis.
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脂肪移植的最佳脂肪处理技术尚未确定。我们已经证明了洗涤吸脂物以去除血液的重要性，但在吸脂过程中没有明显出血时洗涤的必要性尚不清楚。
本研究旨在从炎症、氧化应激和细胞凋亡的角度进一步探讨洗涤对脂肪移植物存活的影响及其潜在机制。
为排除血液的影响，从脂肪抽吸物中分离出去红细胞的下渗液（dEI）。用棉垫过滤分离出的纯化脂肪，与沉降后的 dEI（沉降组）、洗涤（洗涤组）或磷酸盐缓冲液（对照组）混合，然后移植到裸鼠皮下。分别在移植后 1 天和 1、3、8 周时取标本。比较各组间体积和重量保留率、组织学检查、 perilipin-1、CD31、CD45 和 Ly6g 的免疫染色、PPAR-γ、C/EBPα、VEGF、bFGF、IL-6、IL10、TNF-α、TGF-β、Bax 和 Bcl-2 的 mRNA 表达，以及 8-iso-PGF2α、IL-6、IL10、TNF-α 和 TGF-β 的蛋白含量。
移植后，洗涤组体积和重量保留率、组织学评分、活脂肪细胞和血管化均得到改善，脂肪生成和血管生成基因表达增加。与沉降组相比，洗涤组炎症较轻，氧化应激和细胞凋亡水平较低。
洗涤吸脂物以消除混合成分可以提高脂肪移植物的存活率，并促进脂肪生成和血管生成，可能通过减轻炎症、减少氧化应激损伤和抑制细胞凋亡。
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