Carlsson P, Olofsson S O, Bondjers G, Darnfors C, Wiklund O, Bjursell G
Nucleic Acids Res. 1985 Dec 20;13(24):8813-26. doi: 10.1093/nar/13.24.8813.
In this paper we describe the isolation of cDNA clones which code for parts of apolipoprotein B (apoB). The clones were obtained by immunoscreening of an expression library (lambda gt 11) derived from a human hepatoma cell line (Hep G2). The relationship between positive clones and apoB was established with immunochemical techniques using polyclonal as well as monoclonal antibodies. Recombinants, expressing nonoverlapping regions of apoB are described, all hybridizing with a very large mRNA (approximately 20,000 bases long). The nucleotide sequence obtained predicts a primary protein structure with a composition suitable for the formation of stretches of an amphipatic alpha-helix.
在本文中，我们描述了编码载脂蛋白B（apoB）部分序列的cDNA克隆的分离。这些克隆是通过对源自人肝癌细胞系（Hep G2）的表达文库（λgt 11）进行免疫筛选获得的。使用多克隆抗体和单克隆抗体，通过免疫化学技术确定了阳性克隆与apoB之间的关系。描述了表达apoB非重叠区域的重组体，它们均与一种非常大的mRNA（约20,000个碱基长）杂交。所获得的核苷酸序列预测了一种一级蛋白质结构，其组成适合于形成两亲性α-螺旋片段。
