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Deoxynucleotide sequence of an insect cDNA codes for an unreported member of the Chironomus thummi globin family.

作者信息

Saffarini D A, Trewitt P M, Castro M, Wejksnora P J, Bergtrom G

出版信息

Biochem Biophys Res Commun. 1985 Dec 17;133(2):641-7. doi: 10.1016/0006-291x(85)90953-2.

Abstract

Synthetic oligonucleotides served as probes to isolate insect globin clones from a Chironomus thummi cDNA bank. The cDNA insert of one clone (pC-S9) was completely sequenced by the dideoxy termination procedure. Beginning at the 5' end of the coding region, the 584 base pair sequence encodes most of an N-terminal hydrophobic signal sequence and the complete sequence for a mature secreted globin, and contains a polyadenylation recognition site 3' to an appropriate stop codon. The inferred amino acid sequence is that of an unreported variant of hemoglobin VIIB. Based on the number of differences between Hb VIIB chains, the pC-S9 gene has been evolutionarily independent longer than the other (two) members of the globin VIIB subfamily.

摘要

相似文献

1
Deoxynucleotide sequence of an insect cDNA codes for an unreported member of the Chironomus thummi globin family.
Biochem Biophys Res Commun. 1985 Dec 17;133(2):641-7. doi: 10.1016/0006-291x(85)90953-2.
2
Multiple clustered genes of the haemoglobin VIIB subfamily of Chironomus thummi thummi (Diptera).
Gene. 1988 Sep 15;69(1):91-100. doi: 10.1016/0378-1119(88)90381-2.

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