Department of Restorative Dentistry, Faculty of Dentistry, Istanbul Medeniyet University, Istanbul, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Kafkas University, Kars, Turkey.
Sci Rep. 2024 Feb 27;14(1):4725. doi: 10.1038/s41598-024-54331-2.
In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and high viscosity glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines, and apoptosis markers. L929 fibroblast cells were cultured, and dental filling materials were applied in two doses (50 and 100 µl). Immunohistochemical staining was performed for experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect the level of TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT. In the light of the data, the examined dental filling materials were effective on increasing the TGF-beta, IL-10, LPO and CAT levels, and decreasing the TNF-alpha, IL-1-beta, and IL-6 levels. The histological micrographs were also support the issues. When the levels of H-score in Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Biocompatibility varies according to the content of the material, the amount of residual monomer, and its solubility. Although all the experimental groups have cytotoxic effects, the least effect is seen in the Omnichroma group.
在这项研究中，我们的目的是通过评估氧化应激参数、促炎和抗炎细胞因子以及凋亡标志物，研究复合树脂、复合体和高粘度玻璃离子水门汀等常用于牙科的修复材料对 L929 成纤维细胞的影响。培养 L929 成纤维细胞，并以 50 和 100 μl 的两种剂量应用牙科填充材料。用 Anti-Bax 和 Anti-Caspase 9 抗体对实验组进行免疫组织化学染色。然后，使用 ELISA 技术检测 TNF-α、TGF-β、IL-1-β、IL-6、IL-10、LPO 和 CAT 的水平。根据数据，所检查的牙科填充材料可有效提高 TGF-β、IL-10、LPO 和 CAT 的水平，降低 TNF-α、IL-1-β 和 IL-6 的水平。组织学显微照片也支持这些问题。当评估 Caspase 9 标记的显微照片中 H 评分的水平时，对照组的平均值低于实验组的平均值。生物相容性取决于材料的含量、残留单体的量及其溶解度。尽管所有实验组都有细胞毒性作用，但在 Omnichroma 组中观察到的影响最小。
