Endodontic Department, School of Dentistry, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Department of Histology and Embryology, Laboratory of Ultrastructure and Tecidual Biology, Biomedical Center, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Sci Rep. 2019 Mar 8;9(1):3933. doi: 10.1038/s41598-019-40365-4.
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The setting time, flow, radiopacity and water solubility were assessed. To the cytotoxicity assay, primary human osteoblast cells were exposed to several dilutions of both materials eluates. MTT assay, apoptosis assay and cell adhesion assay were performed. The in vivo biocompatibility was evaluated through histological analysis using different staining techniques. No differences were observed between MTA HP and WMTA for setting time, radiopacity, solubility and water absorption (P > 0.05). However, MTA HP showed a significantly higher flow when compared to WMTA (P < 0.05). Cell viability results revealed that the extracts of WMTA and MTA HP promoted the viability of osteoblasts. After incubation of cells with the endodontic cement extracts, the percentage of apoptotic or necrotic cells was very low (<3%). Furthermore, SEM results showed a high degree of cell proliferation and adhesion on both groups. MTA HP showed similar in vivo biocompatibility to the WMTA and the control group in all time-points. The MTA HP presented adequate physicochemical and biological properties with improved flow ability when compared to WMTA. Such improved flow ability may be a result of the addition of a plasticizing agent and should be related to an improvement in the handling of MTA HP.
本研究旨在评估 MTA Repair HP（MTA HP）和 White MTA（WMTA）的理化性能、细胞毒性和体内生物相容性。测定了凝固时间、流动性、射线阻射性和水溶性。将原代人成骨细胞暴露于两种材料浸提液的不同稀释度中，进行细胞毒性试验。采用 MTT 试验、细胞凋亡试验和细胞黏附试验进行检测。通过不同染色技术的组织学分析评价体内生物相容性。MTA HP 和 WMTA 的凝固时间、射线阻射性、溶解度和吸水率无差异（P>0.05）。然而，与 WMTA 相比，MTA HP 的流动性显著更高（P<0.05）。细胞活力结果表明，WMTA 和 MTA HP 的浸提液促进了成骨细胞的活力。细胞与牙髓水泥浸提液孵育后，凋亡或坏死细胞的比例非常低（<3%）。此外，SEM 结果显示两组细胞的增殖和黏附程度较高。在所有时间点，MTA HP 与 WMTA 和对照组的体内生物相容性相似。与 WMTA 相比，MTA HP 具有良好的理化性能和生物相容性，且流动性更好。这种更好的流动性可能是由于添加了增塑剂所致，并且与 MTA HP 操作性能的提高有关。
