Zhong Chao, Li Zhi-Xi, Yang Ling-Jing, Wu Gang, Xiang Bo, Wang Yu-Lan, Zhou Qing
Department of Traditional Chinese Medicine of Orthopedic and Traumatic, Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu City, Sichuan Province, China.
Department of Respiratory Medicine, East Hospital, Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu City, Sichuan Province, China.
Arch Med Sci. 2021 Mar 26;20(1):267-279. doi: 10.5114/aoms/114039. eCollection 2024.
The gene encoding a protein that can regulate the integrin αvβ6 heterodimer protein expression in different status was shown to play an important role in multiple human cancers, such as brain cancer, colon cancer and oral cancer, and is related to clinical progression. This study aims to explore the function and the mechanism of the gene or protein in pancreatic cancer.
We examined the expression of in pancreatic cancer using immunohistochemistry and analyzed the relationship between the expression of and the clinicopathologic features in pancreatic cancer patients. In addition, a bioinformatic method was used to analyze the mRNA level in pancreatic tumor tissues compared with normal pancreatic tissues and to analyze the correlation between high KIF23 expression and prognosis in pancreatic cancer patients. Moreover, colony formation assay, MTT assay, cell scratch, cell invasion and western blot assays and a xenograft mouse model were performed to analyze the effect of KIF23 on proliferation and invasion of pancreatic cancer cells.
Increased expression of was significantly correlated with poor clinical outcome in both our clinical data and TCGA data of pancreatic cancer. Furthermore, functional assays revealed that knockdown and might inhibit cancer cell proliferation and the ability of invasion or migration.
Our data suggest that is associated with pancreatic cancer malignant progression. Hence, may serve as a potential target of pancreatic cancer for future research, and further study is needed.
编码一种能在不同状态下调节整合素αvβ6异二聚体蛋白表达的蛋白质的基因，在多种人类癌症中发挥重要作用，如脑癌、结肠癌和口腔癌，且与临床进展相关。本研究旨在探讨该基因或蛋白质在胰腺癌中的功能及机制。
我们采用免疫组织化学检测胰腺癌中该基因的表达，并分析其表达与胰腺癌患者临床病理特征之间的关系。此外，运用生物信息学方法分析胰腺癌组织与正常胰腺组织中该基因的mRNA水平，并分析该基因高表达与胰腺癌患者预后的相关性。而且，进行了集落形成试验、MTT试验、细胞划痕试验、细胞侵袭试验、蛋白质免疫印迹试验以及异种移植小鼠模型试验，以分析该基因对胰腺癌细胞增殖和侵袭的影响。
在我们的临床数据以及胰腺癌的TCGA数据中，该基因表达增加均与不良临床结局显著相关。此外，功能试验表明，该基因敲低可能抑制癌细胞增殖以及侵袭或迁移能力。
我们的数据表明，该基因与胰腺癌的恶性进展相关。因此，该基因可能成为未来胰腺癌研究的潜在靶点，尚需进一步研究。
