Liu Junru, Rayes Danny, Akbari Omar S
Department of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
GEN Biotechnol. 2024 Feb 1;3(1):35-44. doi: 10.1089/genbio.2023.0041. Epub 2024 Feb 15.
Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.
昆虫基因工程的最新进展为控制害虫和疾病传播媒介种群提供了替代性的基因生物防治解决方案。虽然已经取得了成功，但对于扩大许多基因生物防治干预措施的规模而言，性别分选仍然存在问题。在此，我们描述了一种用于雌雄选择的基因稳定性别分选技术的开发，并在名为SEPARATOR（由转基因可观察报告基因的替代RNA剪接产生的性别分选元件）中进行了概念验证。这种巧妙的方法利用显性表达的荧光蛋白和差异剪接的内含子来确保性别特异性表达。该系统具有适应各种昆虫物种的潜力，并可用于高通量昆虫性别分选。
