School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK.
Anal Methods. 2024 Mar 7;16(10):1454-1467. doi: 10.1039/d3ay02040a.
Saccharides are not only the basic constituents and nutrients of living organisms, but also participate in various life activities, and play important roles in cell recognition, immune regulation, development, cancer, The analysis of carbohydrates and glycoconjugates is a necessary means to study their transformations and physiological roles in living organisms. Existing detection techniques can hardly meet the requirements for the analysis of carbohydrates and glycoconjugates in complex matrices as they are expensive, involve complex derivatization, and are time-consuming. Nanopore sensing technology, which is amplification-free and label-free, and is a high-throughput process, provides a new solution for the identification and sequencing of carbohydrates and glycoconjugates. This review highlights recent advances in novel nanopore-based single-molecule sensing technologies for the detection of carbohydrates and glycoconjugates and discusses the advantages and challenges of nanopore sensing technologies. Finally, current issues and future perspectives are discussed with the aim of improving the performance of nanopores in complex media diagnostic applications, as well as providing a new direction for the quantification of glycan chains and the study of glycan chain properties and functions.
糖不仅是生物的基本组成部分和营养物质，还参与各种生命活动，并在细胞识别、免疫调节、发育、癌症等方面发挥重要作用。分析碳水化合物和糖缀合物是研究它们在生物体内转化和生理作用的必要手段。现有的检测技术由于昂贵、涉及复杂的衍生化和耗时，很难满足复杂基质中碳水化合物和糖缀合物分析的要求。纳米孔传感技术无扩增、无标记，是一种高通量的过程，为碳水化合物和糖缀合物的识别和测序提供了新的解决方案。本文综述了基于纳米孔的新型单分子传感技术在检测碳水化合物和糖缀合物方面的最新进展，并讨论了纳米孔传感技术的优缺点。最后，讨论了当前的问题和未来的展望，以期改善纳米孔在复杂介质诊断应用中的性能，并为聚糖链的定量以及聚糖链性质和功能的研究提供新的方向。
