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中心粒间纤维作为中心粒卫星的附着点，参与纤毛组装。

The intercentriolar fibers function as docking sites of centriolar satellites for cilia assembly.

机构信息

Department of Biological Sciences, Seoul National University, Seoul, South Korea.

出版信息

J Cell Biol. 2024 Apr 1;223(4). doi: 10.1083/jcb.202105065. Epub 2024 Feb 28.

DOI:10.1083/jcb.202105065
PMID:38416111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10901237/
Abstract

Two mother centrioles in an animal cell are linked by intercentriolar fibers that have CROCC/rootletin as their main building block. Here, we investigated the regulatory role of intercentriolar/rootlet fibers in cilia assembly. The cilia formation rates were significantly reduced in the CEP250/C-NAP1 and CROCC/rootletin knockout (KO) cells, irrespective of the departure of the young mother centrioles from the basal bodies. In addition, centriolar satellites were dispersed throughout the cytoplasm in the CEP250 and CROCC KO cells. We observed that PCM1 directly binds to CROCC. Their interaction is critical not only for the accumulation of centriolar satellites near the centrosomes/basal bodies but also for cilia formation. Finally, we observed that the centriolar satellite proteins are localized at the intercentriolar/rootlet fibers in the kidney epithelial cells. Based on these findings, we propose that the intercentriolar/rootlet fibers function as docking sites for centriolar satellites near the centrosomes/basal bodies and facilitate the cilia assembly process.

摘要

在动物细胞中，两个中心体由连接它们的中心粒间纤维相连，该纤维的主要结构基因为 CROCC/根蛋白。在这里，我们研究了中心粒间/根丝纤维在纤毛组装中的调节作用。CEP250/C-NAP1 和 CROCC/根蛋白敲除 (KO) 细胞的纤毛形成率显著降低，而年轻的母中心体是否从基底体外离开并不重要。此外，CEP250 和 CROCC KO 细胞中的中心粒卫星分散在细胞质中。我们观察到 PCM1 直接与 CROCC 结合。它们的相互作用不仅对靠近中心体/基底体的中心粒卫星的积累很重要，而且对纤毛的形成也很重要。最后，我们观察到中心粒卫星蛋白位于肾上皮细胞的中心粒间/根丝纤维中。基于这些发现，我们提出中心粒间/根丝纤维作为靠近中心体/基底体的中心粒卫星的停靠点，有助于纤毛组装过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/7c6589a5539b/JCB_202105065_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/306f82870e1a/JCB_202105065_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/8b0fcc528d73/JCB_202105065_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/4f2bfd7185ba/JCB_202105065_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/dec5ed58ab29/JCB_202105065_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/8222ff9b5269/JCB_202105065_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/cd5e4b8933cc/JCB_202105065_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/575d3ee7f3cd/JCB_202105065_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/0257c2f768e8/JCB_202105065_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/bd913dfe5c3a/JCB_202105065_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/2cc3efb871bd/JCB_202105065_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/22157ffbca12/JCB_202105065_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/7c6589a5539b/JCB_202105065_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/306f82870e1a/JCB_202105065_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/8b0fcc528d73/JCB_202105065_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/4f2bfd7185ba/JCB_202105065_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/dec5ed58ab29/JCB_202105065_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/8222ff9b5269/JCB_202105065_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/cd5e4b8933cc/JCB_202105065_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/575d3ee7f3cd/JCB_202105065_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/0257c2f768e8/JCB_202105065_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/bd913dfe5c3a/JCB_202105065_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/2cc3efb871bd/JCB_202105065_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/22157ffbca12/JCB_202105065_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31e8/10901237/7c6589a5539b/JCB_202105065_Fig7.jpg

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bioRxiv. 2024 Sep 22:2024.06.27.600953. doi: 10.1101/2024.06.27.600953.
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How the centriole builds its cilium: of mothers, daughters, and the acquisition of appendages.中心粒如何构建纤毛：母亲、女儿和附属物的获得。
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