Department of Biological Sciences, Seoul National University, Seoul, Korea.
PLoS One. 2012;7(10):e48196. doi: 10.1371/journal.pone.0048196. Epub 2012 Oct 24.
Centriolar satellites are PCM-1-positive granules surrounding centrosomes. Proposed functions of the centriolar satellites include protein targeting to the centrosome, as well as communication between the centrosome and surrounding cytoplasm. CEP90 is a centriolar satellite protein that is critical for spindle pole integrity in mitotic cells. In this study, we examined the biological functions of CEP90 in interphase cells. CEP90 physically interacts with PCM-1 at centriolar satellites, and this interaction is essential for centrosomal accumulation of the centriolar satellites and eventually for primary cilia formation. CEP90 is also required for BBS4 loading on centriolar satellites and its localization in primary cilia. Our results imply that the assembly and transport of centriolar satellites are critical steps for primary cilia formation and ciliary protein recruitment.
中心体卫星是围绕着中心体的 PCM-1 阳性颗粒。中心体卫星的功能包括将蛋白质靶向到中心体，以及在中心体和周围细胞质之间进行通讯。CEP90 是一种中心体卫星蛋白，对于有丝分裂细胞中纺锤体极的完整性至关重要。在这项研究中，我们研究了 CEP90 在间期细胞中的生物学功能。CEP90 在中心体卫星上与 PCM-1 发生物理相互作用，这种相互作用对于中心体卫星的中心体积累和最终的初级纤毛形成是必不可少的。CEP90 对于 BBS4 在中心体卫星上的加载及其在初级纤毛中的定位也是必需的。我们的结果表明，中心体卫星的组装和运输是初级纤毛形成和纤毛蛋白募集的关键步骤。
