• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

声波诱导的贴壁细胞频闪光学机械成型

Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells.

作者信息

Combriat Thomas, Olsen Petter Angell, Låstad Silja Borring, Malthe-Sørenssen Anders, Krauss Stefan, Dysthe Dag Kristian

机构信息

Njord Centre, Department of Physics, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway.

Hybrid Technology Hub, University of Oslo, Institute of Basic Medical Sciences P.O. Box 1110 Blindern, Oslo, 0317, Norway.

出版信息

Adv Sci (Weinh). 2024 Apr;11(16):e2307929. doi: 10.1002/advs.202307929. Epub 2024 Feb 28.

DOI:10.1002/advs.202307929
PMID:38417124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11040383/
Abstract

In this study, a novel, high content technique using a cylindrical acoustic transducer, stroboscopic fast imaging, and homodyne detection to recover the mechanical properties (dynamic shear modulus) of living adherent cells at low ultrasonic frequencies is presented. By analyzing the micro-oscillations of cells, whole populations are simultaneously mechanotyped with sub-cellular resolution. The technique can be combined with standard fluorescence imaging allowing to further cross-correlate biological and mechanical information. The potential of the technique is demonstrated by mechanotyping co-cultures of different cell types with significantly different mechanical properties.

摘要

在本研究中，提出了一种新颖的高内涵技术，该技术使用圆柱形声换能器、频闪快速成像和零差检测，以在低超声频率下恢复活贴壁细胞的机械性能（动态剪切模量）。通过分析细胞的微振荡，对整个细胞群体同时进行亚细胞分辨率的机械分型。该技术可与标准荧光成像相结合，从而进一步交叉关联生物学和机械信息。通过对具有显著不同机械性能的不同细胞类型的共培养物进行机械分型，证明了该技术的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/5eb21dbf01e2/ADVS-11-2307929-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/6e18dcf6344d/ADVS-11-2307929-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/e400a72b4afe/ADVS-11-2307929-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/489a25bac9cb/ADVS-11-2307929-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/b65838e5fc0d/ADVS-11-2307929-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/7d51f91d8e68/ADVS-11-2307929-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/5eb21dbf01e2/ADVS-11-2307929-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/6e18dcf6344d/ADVS-11-2307929-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/e400a72b4afe/ADVS-11-2307929-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/489a25bac9cb/ADVS-11-2307929-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/b65838e5fc0d/ADVS-11-2307929-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/7d51f91d8e68/ADVS-11-2307929-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04c2/11040383/5eb21dbf01e2/ADVS-11-2307929-g005.jpg

相似文献

1
Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells.声波诱导的贴壁细胞频闪光学机械成型
Adv Sci (Weinh). 2024 Apr;11(16):e2307929. doi: 10.1002/advs.202307929. Epub 2024 Feb 28.
2
Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection.
Appl Opt. 2006 Mar 20;45(9):2115-23. doi: 10.1364/ao.45.002115.
3
Imaging the cellular response to transient shear stress using stroboscopic digital holography.使用频闪数字全息术对瞬时剪切应力下的细胞响应进行成像。
J Biomed Opt. 2011 Dec;16(12):120508. doi: 10.1117/1.3665441.
4
Three-dimensional vibrometry of the human eardrum with stroboscopic lensless digital holography.采用频闪无透镜数字全息术对人鼓膜进行三维振动测量。
J Biomed Opt. 2015 May;20(5):051028. doi: 10.1117/1.JBO.20.5.051028.
5
Motion of the tympanic membrane after cartilage tympanoplasty determined by stroboscopic holography.声反射描记术观察鼓膜修补术后鼓膜运动
Hear Res. 2010 May;263(1-2):78-84. doi: 10.1016/j.heares.2009.11.005. Epub 2009 Nov 10.
6
High-speed stroboscopic imaging with frequency-doubled supercontinuum.基于倍频超连续谱的高速频闪成像。
Opt Lett. 2013 Mar 1;38(5):658-60. doi: 10.1364/OL.38.000658.
7
All-optical broadband ultrasonography of single cells.单细胞的全光宽带超声成像
Sci Rep. 2015 Mar 3;5:8650. doi: 10.1038/srep08650.
8
Imaging deformation of adherent cells due to shear stress using quantitative phase imaging.使用定量相成像技术对粘附细胞因剪切应力产生的形变进行成像。
Opt Lett. 2016 Jan 15;41(2):352-5. doi: 10.1364/OL.41.000352.
9
Biomechanics of C2C12 Cells Observed with Cellular Resolution Scanning Acoustic Microscope Combined with Optical Microscope.结合光学显微镜使用细胞分辨率扫描声学显微镜观察C2C12细胞的生物力学特性
Annu Int Conf IEEE Eng Med Biol Soc. 2019 Jul;2019:4828-4831. doi: 10.1109/EMBC.2019.8857008.
10
Stroboscopic ultrahigh-resolution full-field optical coherence tomography.频闪超高分辨率全场光学相干断层扫描
Opt Lett. 2005 Jun 1;30(11):1351-3. doi: 10.1364/ol.30.001351.

本文引用的文献

1
When seeing is not believing: application-appropriate validation matters for quantitative bioimage analysis.眼见不一定为实：定量生物图像分析中与应用相匹配的验证至关重要。
Nat Methods. 2023 Jul;20(7):968-970. doi: 10.1038/s41592-023-01881-4.
2
Frequency-dependent transition in power-law rheological behavior of living cells.活细胞幂律流变行为中的频率依赖性转变。
Sci Adv. 2022 May 6;8(18):eabn6093. doi: 10.1126/sciadv.abn6093.
3
Effects of extracellular matrix viscoelasticity on cellular behaviour.细胞外基质粘弹性对细胞行为的影响。
Nature. 2020 Aug;584(7822):535-546. doi: 10.1038/s41586-020-2612-2. Epub 2020 Aug 26.
4
Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction.细胞黏附复合体在机械转导调控中的相互作用
Bioessays. 2020 Nov;42(11):e2000119. doi: 10.1002/bies.202000119. Epub 2020 Aug 23.
5
Variations of Elastic Modulus and Cell Volume with Temperature for Cortical Neurons.皮质神经元的弹性模量和细胞体积随温度的变化。
Langmuir. 2019 Aug 20;35(33):10965-10976. doi: 10.1021/acs.langmuir.9b01651. Epub 2019 Aug 9.
6
A comparison of methods to assess cell mechanical properties.细胞力学特性评估方法的比较。
Nat Methods. 2018 Jul;15(7):491-498. doi: 10.1038/s41592-018-0015-1. Epub 2018 Jun 18.
7
Separate roles for chromatin and lamins in nuclear mechanics.染色质和核纤层在核力学中的分离作用。
Nucleus. 2018 Jan 1;9(1):119-124. doi: 10.1080/19491034.2017.1414118. Epub 2017 Dec 28.
8
Mechanical Properties of the Cytoskeleton and Cells.细胞骨架和细胞的力学性质
Cold Spring Harb Perspect Biol. 2017 Nov 1;9(11):a022038. doi: 10.1101/cshperspect.a022038.
9
High-frequency microrheology reveals cytoskeleton dynamics in living cells.高频微观流变学揭示活细胞中的细胞骨架动力学。
Nat Phys. 2017 Aug;13(8):771-775. doi: 10.1038/nphys4104. Epub 2017 May 1.
10
Cell stiffness determined by atomic force microscopy and its correlation with cell motility.通过原子力显微镜测定的细胞硬度及其与细胞运动性的相关性。
Biochim Biophys Acta. 2016 Sep;1860(9):1953-60. doi: 10.1016/j.bbagen.2016.06.010. Epub 2016 Jun 8.