Anudit Chanihcha, Saraisuwan Pornthip, Kimterng Chantana, Puangmanee Chanakan, Bamphensin Nicharee, Kerdsin Anusak
Microbiology Unit, Uthai Thani Hospital, Thailand.
Faculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Thailand.
Jpn J Infect Dis. 2024 Jul 23;77(4):220-226. doi: 10.7883/yoken.JJID.2023.376. Epub 2024 Feb 29.
Escherichia coli is a Gram-negative bacterium that causes a variety of clinical infections in humans, including diarrhea, sepsis, and urinary tract infection. This bacterium is a common multidrug-resistant threat in community and hospital settings worldwide. This study examined the antimicrobial susceptibility and genetic relationship based on Clermont phylotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR of 84 E. coli urinary isolates from provincial and community hospitals in Thailand. All isolates were susceptible to nitrofurantoin, and almost all isolates were susceptible to carbapenem, fosfomycin, and amikacin. High resistance rates to fluoroquinolone, ampicillin, and trimethoprim/sulfamethoxazole were observed. Clermont phylogroup B2 was predominant (n = 58). Subtyping of the B2 phylogroup revealed diverse subgroups, of which subgroup V (n = 11), VII (n = 9), III (n = 6), and II (n = 6) were most prevalent. ERIC-PCR showed that the strains of the B2 subgroups III and V were spread between provincial and community hospitals and between hospital wards. This evidence suggests the need for comprehensive infection control monitoring, with strong active surveillance at all hospital levels.
大肠杆菌是一种革兰氏阴性菌，可导致人类多种临床感染，包括腹泻、败血症和尿路感染。这种细菌是全球社区和医院环境中常见的多重耐药威胁。本研究基于克莱蒙分型法和肠杆菌重复基因间共识（ERIC）-PCR，对泰国省级和社区医院的84株大肠杆菌尿液分离株进行了抗菌药敏性和遗传关系研究。所有分离株对呋喃妥因敏感，几乎所有分离株对碳青霉烯类、磷霉素和阿米卡星敏感。观察到对氟喹诺酮类、氨苄西林和甲氧苄啶/磺胺甲恶唑的高耐药率。克莱蒙B2菌群占主导地位（n = 58）。B2菌群的亚型分析显示出不同的亚群，其中V亚群（n = 11）、VII亚群（n = 9）、III亚群（n = 6）和II亚群（n = 6）最为常见。ERIC-PCR显示，B2亚群III和V的菌株在省级和社区医院之间以及医院病房之间传播。这一证据表明需要进行全面的感染控制监测，并在各级医院进行强有力的主动监测。
