Sattasathuchana Panpicha, Srikullabutr Suttiporn, Kerdsin Anusak, Assawarachan Sathidpak Nantasanti, Amavisit Patamabhorn, Surachetpong Win, Thengchaisri Naris
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Department of Community Health, Faculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.
BMC Vet Res. 2024 Dec 20;20(1):573. doi: 10.1186/s12917-024-04435-8.
Antimicrobial resistance (AMR) is a global health concern that is exacerbated by the transmission of bacteria and genetic material between humans, animals and the environment. This study investigated AMR of Escherichia coli (E. coli) isolated from cats' feces and their drinking water. The study compared the AMR of fecal and environmental E. coli isolates from pet cats.
A total of 104 samples (52 cat feces and 52 cat drinking water samples) was cultured for E. coli. The study compared the AMR of fecal and environmental E. coli isolates from pet cats. An analysis of carbapenemase and extended-spectrum β-lactamase (ESBL)-producing E. coli genes (bla, bla and bla) and phylogroups of E. coli was also performed. E. coli was identified from all fecal (100%) and almost half of drinking water (44.2%) samples. All E. coli isolate was susceptible to amikacin or imipenem. Clindamycin showed the highest resistance rate. β-lactam was the most found with co-resistance profiles, comprising β-lactams with aminoglycosides, quinolones, sulfonamides, macrolides or carbapenems. Very strong positive correlations of bactericidal agents were found among quinolones (r > 0.8, p < 0.01). Within the group of bacteriostatic agents, moderate correlation was observed between azithromycin and sulfa-trimethoprim (r = 0.5253, p < 0.01). Carbapenemase gene was not detected in this study. Extended-spectrum β-lactamase-producing E. coli genes (bla, bla and bla) were identified in E. coli isolates, with bla being the most predominant. Furthermore, phylogroup B2 was the dominant segregation among the E. coli, particularly in fecal isolates.
This study identified AMRin E. coli isolated from cats' feces and their drinking water. The results revealed that the phylogroup B2 was predominant, with bla being the most widespread ESBL gene.
抗菌药物耐药性（AMR）是一个全球卫生问题，人类、动物和环境之间细菌及遗传物质的传播加剧了这一问题。本研究调查了从猫粪便及其饮用水中分离出的大肠杆菌（E. coli）的AMR情况。该研究比较了宠物猫粪便和环境中大肠杆菌分离株的AMR情况。
共培养了104份样本（52份猫粪便和52份猫饮用水样本）以检测大肠杆菌。该研究比较了宠物猫粪便和环境中大肠杆菌分离株的AMR情况。还对产碳青霉烯酶和超广谱β-内酰胺酶（ESBL）的大肠杆菌基因（bla、bla和bla）以及大肠杆菌的系统发育群进行了分析。在所有粪便样本（100%）和几乎一半的饮用水样本（44.2%）中都鉴定出了大肠杆菌。所有大肠杆菌分离株对阿米卡星或亚胺培南敏感。克林霉素显示出最高的耐药率。β-内酰胺类药物与其他药物的联合耐药情况最为常见，包括β-内酰胺类药物与氨基糖苷类、喹诺酮类、磺胺类、大环内酯类或碳青霉烯类药物。在喹诺酮类药物之间发现了很强的杀菌剂正相关性（r > 0.8，p < 0.01）。在抑菌剂组中，阿奇霉素和磺胺甲恶唑之间观察到中等相关性（r = 0.5253，p < 0.01）。本研究未检测到碳青霉烯酶基因。在大肠杆菌分离株中鉴定出了产超广谱β-内酰胺酶的大肠杆菌基因（bla、bla和bla），其中bla最为常见。此外，系统发育群B2是大肠杆菌中的主要分类，特别是在粪便分离株中。
本研究鉴定了从猫粪便及其饮用水中分离出的大肠杆菌的AMR情况。结果显示系统发育群B2占主导地位，bla是分布最广泛的ESBL基因。
