使用流式细胞术检测小鼠结膜和泪腺中免疫亚群的方案。
Protocol to examine immune subpopulations in murine conjunctiva and lacrimal gland using flow cytometry.
机构信息
Department of Ophthalmology, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing 100191, China.
Department of Ophthalmology, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing 100191, China.
出版信息
STAR Protoc. 2024 Mar 15;5(1):102921. doi: 10.1016/j.xpro.2024.102921. Epub 2024 Feb 28.
Here, we present a protocol for the examination of immune cells in the murine conjunctiva and lacrimal gland using flow cytometry. We describe steps for dissection, preparation of high-quality single-cell suspensions, utilization of comprehensive staining panels, and optimization of flow cytometry voltage. We then detail procedures for compensation adjustments and the implementation of effective gating strategies. For complete details on the use and execution of this protocol, please refer to Ma et al..
在这里，我们提供了一个使用流式细胞术检查小鼠结膜和泪腺中免疫细胞的方案。我们描述了解剖、制备高质量单细胞悬浮液、利用全面染色面板以及优化流式细胞术电压的步骤。然后，我们详细介绍了补偿调整和实施有效门控策略的程序。有关使用和执行此方案的完整详细信息，请参考 Ma 等人的研究。
Zotero 插件