使用基于荧光的流式细胞术检测小鼠内脏脂肪组织免疫细胞的方案。
Protocol to examine murine visceral adipose tissue immune cells using fluorescence-based flow cytometry.
机构信息
Molecular Pharmacology and Therapeutics Graduate Program, Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA; Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Molecular Pharmacology and Therapeutics Graduate Program, Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA; Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
出版信息
STAR Protoc. 2024 Sep 20;5(3):103227. doi: 10.1016/j.xpro.2024.103227. Epub 2024 Aug 10.
Adipose tissue immune cells are heterogeneous and dynamic, alter metabolism, and drive immune responses. Here, we present a protocol for assessment and characterization of murine adipose tissue immune cells using fluorescence-based flow cytometry and sorting into pure populations. We describe steps for isolation of the stromovascular fraction, antibody staining, and data collection by flow cytometry. We also discuss common issues and troubleshooting steps. For complete details on the use and execution of this protocol, please refer to Carey et al..
脂肪组织免疫细胞具有异质性和动态性，可改变代谢并驱动免疫反应。在这里，我们提出了一种使用基于荧光的流式细胞术和分选成纯群体来评估和表征小鼠脂肪组织免疫细胞的方案。我们描述了分离基质血管部分、抗体染色和流式细胞术数据收集的步骤。我们还讨论了常见问题和故障排除步骤。有关使用和执行此方案的完整详细信息，请参阅 Carey 等人的研究。
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