Moores Cancer Center, University of California, San Diego, La Jolla, CA 92037, USA.
Moores Cancer Center, University of California, San Diego, La Jolla, CA 92037, USA; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92037, USA.
STAR Protoc. 2024 Sep 20;5(3):103139. doi: 10.1016/j.xpro.2024.103139. Epub 2024 Jun 14.
Flow cytometry, single-cell RNA sequencing, and other analyses enable us to capture immune profiles of the tumor microenvironment. Here, we present a protocol to characterize the immune profile of tumor-bearing mice. We describe steps for establishing mouse models and preparing single-cell suspensions from tumor tissue and other immune-related organs, which can be further analyzed by flow cytometry and other omics assays. We then detail procedures for staining, flow cytometry analysis, and phenotyping of the immune cell populations. For complete details on the use and execution of this protocol, please refer to Miyauchi et al..
流式细胞术、单细胞 RNA 测序和其他分析方法使我们能够捕获肿瘤微环境的免疫特征。在这里，我们提出了一种方案来描述肿瘤小鼠的免疫特征。我们描述了建立小鼠模型和从肿瘤组织和其他免疫相关器官中制备单细胞悬液的步骤，这些细胞悬液可以进一步通过流式细胞术和其他组学分析进行分析。然后，我们详细介绍了免疫细胞群体的染色、流式细胞术分析和表型分析的步骤。有关该方案使用和执行的完整详细信息，请参考 Miyauchi 等人的研究。
