流式细胞术分析小鼠角蛋白细胞和皮肤免疫细胞亚群。
Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells.
机构信息
Cutaneous Leukocyte Biology Section, Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.
出版信息
STAR Protoc. 2021 Dec 16;3(1):101052. doi: 10.1016/j.xpro.2021.101052. eCollection 2022 Mar 18.
Skin is our body's outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).
皮肤是人体最外层的物理屏障，也是富含各种免疫细胞和非免疫细胞的免疫界面。然而，高效地生成用于流式细胞术分析的单细胞悬液可能具有挑战性。在此，我们提供了获取表皮和全层皮肤细胞悬液的方案，并提供了通过流式细胞术鉴定小鼠角质形成细胞和皮肤免疫细胞亚群的门控策略。如需了解本方案使用和实施的详细信息，请参考 Sakamoto 等人（2021 年）。
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