Intajak Papichaya, Yuan Yuan, Sakaguchi Norihito, Saikaew Pipop, Eamsaard Pimpinee, Matsumoto Mariko, Sano Hidehiko, Tomokiyo Atsushi
Department of Restorative Dentistry, Graduate School of Dental Medicine, Hokkaido University, Kita 8, Nishi 5, Kita-ku, Sapporo, 060-080, Japan.
Center of Advanced Research of Energy and Materials, Faculty of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Dent Mater. 2024 Apr;40(4):e24-e32. doi: 10.1016/j.dental.2024.02.020. Epub 2024 Feb 28.
This study aimed to evaluate microtensile bond strength (µTBS) and ultra-morphological characteristic changes in sound dentin resulting from silver diamine fluoride (SDF) when using adhesives.
Ninety-six extracted human third molars were divided into the SDF-contaminated dentin group and the sound dentin group. In the SDF-contaminated dentin group, 38% SDF was agitated for 1 min, left undisturbed for 2 min, and rinsed with distilled water for 30 s. Then, each group was further subdivided into six subgroups (n = 8 / group) according to application modes: self-etch mode (SE) and etch-and-rinse mode (ER) followed by three adhesives: (1) Scothbond Universal Plus Adhesive (SUP); (2) G2-Bond Universal Adhesive (G2B); and (3) Clearfil Mega Bond 2 (MB2). All specimens were restored with resin composite and were stored in distilled water for 24 h before μTBS testing. Data from the μTBS test were analyzed using Three-way ANOVA and Duncan test (p < 0.05). The morphology of fractured surface and adhesive-dentin interfaces were evaluated by SEM, TEM, and STEM. Further elemental analysis was done by EDX.
All SDF-contaminated dentin groups demonstrated significantly lower μTBS than sound dentin groups. All ER groups had higher μTBS than SE groups, except for G2B in the sound dentin group. STEM/EDX revealed an SDF-dentin-reacted layer in the SDF-contaminated dentin group.
SDF had an adverse effect on adhesives. Additionally, ER mode is preferable when bonded to SDF-contaminated dentin. A calcium and fluoride-contained layer was observed in all SDF-contaminated dentin.
本研究旨在评估使用粘结剂时，氟化银（SDF）对健康牙本质微拉伸粘结强度（µTBS）及超微形态特征变化的影响。
将96颗拔除的人第三磨牙分为SDF污染牙本质组和健康牙本质组。在SDF污染牙本质组中，将38%的SDF搅拌1分钟，静置2分钟，然后用蒸馏水冲洗30秒。然后，根据应用模式将每组进一步细分为六个亚组（每组n = 8）：自酸蚀模式（SE）和酸蚀冲洗模式（ER），随后使用三种粘结剂：（1）Scothbond Universal Plus粘结剂（SUP）；（2）G2-Bond通用粘结剂（G2B）；（3）Clearfil Mega Bond 2（MB2）。所有标本均用树脂复合材料修复，并在进行µTBS测试前在蒸馏水中保存24小时。使用三因素方差分析和邓肯检验（p < 0.05）分析µTBS测试数据。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和扫描透射电子显微镜（STEM）评估断裂表面和粘结剂-牙本质界面的形态。通过能谱仪（EDX）进行进一步的元素分析。
所有SDF污染牙本质组的µTBS均显著低于健康牙本质组。除健康牙本质组中的G2B外，所有ER组的µTBS均高于SE组。STEM/EDX显示SDF污染牙本质组中有SDF-牙本质反应层。
SDF对粘结剂有不利影响。此外，与SDF污染牙本质粘结时，ER模式更可取。在所有SDF污染牙本质中均观察到含钙和氟的层。
