Department of Restorative Dentistry, Faculty of Dental Medicine, Hokkaido University, Kita 8, Nishi 5, Kita-ku, Sapporo 060-080, Japan.
Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University, 114 Sukhumvit 23, Wattana, Bangkok 10110, Thailand.
Dent Mater. 2024 Nov;40(11):2034-2042. doi: 10.1016/j.dental.2024.09.015. Epub 2024 Oct 2.
This study aims to investigate the effect of colloidal platinum nanoparticles (CPN) on the durability of resin-dentin bonding performance with contemporary adhesives.
Sixty non-carious human maxillary premolars were subjected to microtensile bond strength (µTBS) testing and divided into two main groups: CPN-treated and untreated. Within each group, specimens were randomly allocated to Clearfil Megabond 2 (MB2), Scotchbond Universal Plus Adhesive with self-etch mode (SE-SUP), and etch-and-rinse mode (ER-SUP) subgroups (n = 10/group). CPN was applied to dentin in the MB2 and SE-SUP groups for 20 s, followed by rinsing before adhesive application. In the ER-SUP group, CPN was applied after etch-and-rinse. The µTBS was tested after 24 h, 6 months, and 1 year, and the fracture modes were observed using SEM. The µTBS data were analyzed using a two-way ANOVA and post-hoc Tukey HSD test (α = 0.05). An additional twelve premolars underwent TEM/STEM/EDX for ultra-morphological observations.
The application of CPN significantly prevented a decline in the µTBS of both the MB2 and SE-SUP groups. No significant decrease was observed in the ER-SUP group, either with aging or CPN application. Ultra-morphological images revealed platinum nanoparticles attaching to the collagen fibrils of the hybrid layer regardless of aging. It was highlighted that the nanoparticles attached to the banded collagen in the aging groups were observed.
CPN exhibits the potential in enhancing the longevity of resin-dentin bonding in SE mode.
本研究旨在探讨胶体铂纳米粒子（CPN）对当代胶粘剂的树脂-牙本质粘结性能耐久性的影响。
60 颗无龋上颌前磨牙进行微拉伸粘结强度（µTBS）测试，分为CPN 处理组和未处理组。每组内随机分为 Clearfil Megabond 2（MB2）、自酸蚀 Scotchbond Universal Plus 胶粘剂（SE-SUP）和全酸蚀 Scotchbond Universal Plus 胶粘剂（ER-SUP）亚组（每组 n=10）。MB2 和 SE-SUP 组的牙本质上应用 CPN 20s，然后在应用胶粘剂前冲洗。ER-SUP 组在酸蚀-冲洗后应用 CPN。24h、6 个月和 1 年后测试 µTBS，并用 SEM 观察断裂模式。采用双因素方差分析和事后 Tukey HSD 检验（α=0.05）分析 µTBS 数据。另外 12 颗前磨牙进行 TEM/STEM/EDX 超微形态学观察。
CPN 的应用显著防止了 MB2 和 SE-SUP 组的 µTBS 下降。老化或 CPN 应用均未观察到 ER-SUP 组的显著下降。超微形态图像显示铂纳米粒子附着在混合层的胶原纤维上，无论老化如何。值得注意的是，在老化组中观察到附着在带纹胶原上的纳米粒子。
CPN 具有增强 SE 模式下树脂-牙本质粘结耐久性的潜力。
