Laboratory of Pharmacology and Molecular Chemistry (LFQM), Department of Biological Chemistry, Regional University of Cariri (URCA), 63105-000, Crato, CE, Brazil.
Oswaldo Cruz Foundation (Fiocruz), Fiocruz Ceará, 60180-900, Eusébio, CE, Brazil.
Chem Biodivers. 2024 Apr;21(4):e202400187. doi: 10.1002/cbdv.202400187. Epub 2024 Mar 20.
Psidium brownianum Mart is reported in the literature by antinociceptive and antioxidant activities, indicating that this species' secondary metabolites might be used to control inflammatory processes. The present study aimed to characterize the topical antiedematogenic activity of the essential oil of Psidium brownianum Mart. (OEPB) in ear edema models by different inflammatory agents. Female Swiss mice (25-35 g) and Wistar albino rats (200-300 g) were used throughout tests (n=6/group) on acute or chronic edema models induced by single and multiple topical applications. The OEPB is administered topically pure or at a concentration of 100 or 200 mg/mL. The antiedematogenic mechanism of OEPB was analyzed by administering capsaicin, arachidonic acid, histamine, and phenol at the best effective dose (200 mg/mL). The results showed a significant reduction of edema-induced single (28.87 %) and multiple (50.13 %) applications of croton oil compared to the negative control group. Regarding potential mechanisms of action, OEPB (200 mg/mL) inhibited the development of edema triggered by capsaicin (29.95 %), arachidonic acid (22.66 %), phenol (23.35 %), and histamine (75.46 %), suggesting an interference with the histaminergic pathway. These results indicate that OEPB presents a topical antiedematogenic effect in acute and chronic murine models, possibly interfering with inflammatory pathways triggered by mediators such as histamine.
文献报道桃金娘科植物番石榴具有镇痛和抗氧化活性，表明该物种的次生代谢产物可能用于控制炎症过程。本研究旨在通过不同的炎症介质来表征番石榴叶挥发油（OEPB）对耳肿胀模型的局部抗水肿活性。雌性瑞士小鼠（25-35g）和 Wistar 白化大鼠（200-300g）用于急性或慢性水肿模型（n=6/组）的测试，这些模型是通过单次和多次局部应用诱导的。OEPB 以纯品或 100 或 200mg/mL 的浓度给药。通过给予辣椒素、花生四烯酸、组胺和苯酚的最佳有效剂量（200mg/mL）来分析 OEPB 的抗水肿机制。结果表明，与阴性对照组相比，番石榴叶挥发油对单次（28.87%）和多次（50.13%）应用巴豆油诱导的水肿有显著抑制作用。关于潜在的作用机制，OEPB（200mg/mL）抑制了辣椒素（29.95%）、花生四烯酸（22.66%）、苯酚（23.35%）和组胺（75.46%）引发的水肿发展，表明其干扰了组胺能途径。这些结果表明，OEPB 对急性和慢性小鼠模型具有局部抗水肿作用，可能通过干扰组胺等介质触发的炎症途径来发挥作用。
