Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Department of Laboratory Medicine, Shanghai Geriatric Medical Center, Zhongshan Hospital, Fudan University, Shanghai, China.
J Cell Mol Med. 2024 Mar;28(6):e18147. doi: 10.1111/jcmm.18147.
HCC is a globally high-incidence malignant tumour, and its pathogenesis is still unclear. Recently, STRN3 has been found to be elevated in various tumours, but its expression and biological functions in HCC have not been studied. In the study, clinical correlation analysis was performed on 371 liver cancer patients from TCGA database and liver cancer tissues and normal tissues from the GEO database. qRT-PCR and western blotting were used to detect relevant proteins in cells, and CCK8 and colony formation experiments were performed to analyse cell proliferation ability. Transwell and wound healing experiments were performed to detect cell invasion ability, and flow cytometry was used to detect cell apoptosis. Single-cell sequencing data and multiple immunofluorescence were analysed for the expression abundance and distribution of certain proteins. Immunohistochemistry was used to assess the expression of STRN3 in patients' tumour and adjacent non-cancerous tissues. The results indicated STRN3 was highly expressed in liver tumour tissues and was closely associated with poor prognosis. Knockdown of STRN3 could significantly inhibit cell proliferation and migration ability. At the same time, we found that STRN3 could inhibit the Hippo pathway and promote the entry of YAP protein into the nucleus. Our study first found that STRN3 could promote tumour growth by inhibiting the Hippo pathway. The study of STRN3 can promote the understanding and treatment of the occurrence and development of HCC.
HCC 是一种全球高发的恶性肿瘤，其发病机制尚不清楚。最近，研究发现 STRN3 在多种肿瘤中升高，但在 HCC 中的表达和生物学功能尚未研究。本研究在 TCGA 数据库中对 371 例肝癌患者和 GEO 数据库中的肝癌组织和正常组织进行临床相关性分析。采用 qRT-PCR 和 Western blot 检测细胞中相关蛋白，CCK8 和集落形成实验分析细胞增殖能力，Transwell 和划痕愈合实验检测细胞侵袭能力，流式细胞术检测细胞凋亡。单细胞测序数据和多重免疫荧光分析某些蛋白的表达丰度和分布。免疫组化评估 STRN3 在患者肿瘤和相邻非癌组织中的表达。结果表明 STRN3 在肝肿瘤组织中高表达，与预后不良密切相关。STRN3 敲低可显著抑制细胞增殖和迁移能力。同时，我们发现 STRN3 可以抑制 Hippo 通路，促进 YAP 蛋白进入细胞核。本研究首次发现 STRN3 可以通过抑制 Hippo 通路促进肿瘤生长。对 STRN3 的研究可以促进对 HCC 发生发展的理解和治疗。
