Department of Animal Physiology, Biochemistry and Biostructure, Poznan University of Life Sciences, Poznan, Poland.
Histochem Cell Biol. 2024 Jun;161(6):449-460. doi: 10.1007/s00418-024-02274-0. Epub 2024 Mar 2.
The aim of this study is to determine the influence of the mitochondrial open-reading-frame of the twelve S rRNA-c (MOTS-c) peptide on pancreatic cell physiology. Moreover, in this study, we examined the changes in MOTS-c secretion and expression under different conditions. Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively. As the pancreas is an endocrine organ, we also tested its hormone regulation capabilities. Furthermore, we assessed the secretion of MOTS-c after incubating the cells with glucose and free fatty acids. Additionally, we examined key cell culture parameters such as cell viability, proliferation, and apoptosis. The results obtained from this study show that MOTS-c has a significant impact on the physiology of pancreatic cells. Specifically, it lowers insulin secretion and expression in INS-1E cells and enhances glucagon secretion and expression in αTC-1 cells. Furthermore, MOTS-c affects cell viability and apoptosis. Interestingly, insulin and glucagon affect the MOTS-c secretion as well as glucose and free fatty acids. These experiments clearly show that MOTS-c is an important regulator of pancreatic metabolism, and there are numerous properties of MOTS-c yet to be discovered.
本研究旨在确定线粒体开放阅读框的十二 S rRNA-c (MOTS-c) 肽对胰腺细胞生理学的影响。此外，在本研究中，我们研究了 MOTS-c 分泌和表达在不同条件下的变化。我们的实验使用了实验室细胞系培养物，特别是 INS-1E 和 αTC-1 细胞系，它们分别代表β和α胰腺细胞。由于胰腺是内分泌器官，我们还测试了其激素调节能力。此外，我们评估了在孵育细胞葡萄糖和游离脂肪酸后 MOTS-c 的分泌。此外，我们还检查了细胞活力、增殖和细胞凋亡等关键细胞培养参数。这项研究的结果表明，MOTS-c 对胰腺细胞的生理学有重大影响。具体来说，它降低了 INS-1E 细胞中胰岛素的分泌和表达，增强了 αTC-1 细胞中胰高血糖素的分泌和表达。此外，MOTS-c 还影响细胞活力和细胞凋亡。有趣的是，胰岛素和胰高血糖素也会影响 MOTS-c 的分泌以及葡萄糖和游离脂肪酸。这些实验清楚地表明，MOTS-c 是胰腺代谢的重要调节剂，而 MOTS-c 还有许多性质有待发现。
