Institute of Crop Science, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China.
Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
Ecotoxicol Environ Saf. 2024 Mar 15;273:116166. doi: 10.1016/j.ecoenv.2024.116166. Epub 2024 Mar 1.
Nanotechnology is one of the most recent approaches employed to defend plants against both biotic and abiotic stress including heavy metals such as Cadmium (Cd). In this study, we evaluated the effects of titanium dioxide (TiO) nanoparticles (TiO NPs) in alleviating Cd stress in Tetrastigma hemsleyanum Diels et Gilg. Compared with Cd treatment, TiO NPs decreased leaf Cd concentration, restored Cd exposure-related reduction in the biomass to about 69% of control and decreased activities of antioxidative enzymes. Integrative analysis of transcriptome and metabolome revealed 325 differentially expressed genes associated with TiO NP treatment, most of which were enriched in biosynthesis of secondary metabolites. Among them, the flavonoid and phenylpropanoid biosynthetic pathways were significantly regulated to improve the growth of T. hemsleyanum when treated with Cd. In the KEGG Markup Language (KGML) network analysis, we found some commonly regulated pathways between Cd and Cd+TiO NP treatment, including phenylpropanoid biosynthesis, ABC transporters, and isoflavonoid biosynthesis, indicating their potential core network positions in controlling T. hemsleyanum response to Cd stress. Overall, our findings revealed a complex response system for tolerating Cd, encompassing the transportation, reactive oxygen species scavenging, regulation of gene expression, and metabolite accumulation in T. hemsleyanum. Our results indicate that TiO NP can be used to reduce Cd toxicity in T. hemsleyanum.
纳米技术是一种用于保护植物免受生物和非生物胁迫的最新方法，包括重金属如镉（Cd）。在这项研究中，我们评估了二氧化钛（TiO）纳米粒子（TiO NPs）在缓解 Tetrastigma hemsleyanum Diels et Gilg 中 Cd 胁迫的作用。与 Cd 处理相比，TiO NPs 降低了叶片 Cd 浓度，将 Cd 暴露相关的生物量减少恢复到对照的约 69%，并降低了抗氧化酶的活性。转录组和代谢组的综合分析揭示了 325 个与 TiO NP 处理相关的差异表达基因，其中大多数富集在次生代谢物的生物合成中。在这些基因中，类黄酮和苯丙烷生物合成途径被显著调控，以改善 Cd 处理下 T. hemsleyanum 的生长。在 KEGG 标记语言（KGML）网络分析中，我们发现 Cd 和 Cd+TiO NP 处理之间存在一些共同调节的途径，包括苯丙烷生物合成、ABC 转运蛋白和异黄酮生物合成，表明它们在控制 T. hemsleyanum 对 Cd 胁迫的反应中具有潜在的核心网络位置。总的来说，我们的研究结果揭示了 Tetrastigma hemsleyanum 耐受 Cd 的复杂响应系统，包括运输、活性氧物种清除、基因表达调控和代谢物积累。我们的结果表明，TiO NP 可用于降低 T. hemsleyanum 中的 Cd 毒性。
