Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Hangzhou 311300, China.
College of Food and Health, Department of Traditional Chinese medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.
Molecules. 2022 Dec 22;28(1):83. doi: 10.3390/molecules28010083.
Diels et Gilg, known as a "plant antibiotic", possesses several attractive properties including anti-inflammatory, anti-tumor, and antioxidant effects, with its efficacy being attributed to flavonoids. However, the flavonoid biosynthesis of has rarely been studied. In this study, we investigated the flavonoid metabolism of through metabolome analysis and transcriptome sequencing. The metabolomic results showed differences in the flavonoids of the leaves and root tubers of . A total of 22 flavonoids was detected, and the concentrations of most flavonoids in the leaves were higher than those in the root tubers. Transcriptome analysis revealed that differentially expressed genes (DEGs) in the leaves and root tubers were enriched in photosynthesis-antenna proteins. Pearson correlation analysis indicated that the expression levels of chalcone isomerase (CHI) and UDP-glycose flavonoid glycosyltransferase (UFGT) were highly correlated with the concentrations of most flavonoids. Further, this study found that the photosynthesis-antenna proteins essentially contributed to the difference in the flavonoids in . The gene expressions and concentrations of the total flavonoids of leaves and root tubers in Hangzhou, Jinhua, Lishui, and Taizhou in Zhejiang Province, China, showed that CHI (CL6715.Contig1_All, Unigene19431_All, CL921.Contig4_All) and UFGT (CL11556.Contig3_All, CL11775.Contig1_All) were the potential key genes of accumulation of most flavonoids in .
Diels et Gilg，被称为“植物抗生素”，具有多种吸引人的特性，包括抗炎、抗肿瘤和抗氧化作用，其功效归因于类黄酮。然而， 的类黄酮生物合成很少被研究。在这项研究中，我们通过代谢组学分析和转录组测序研究了 的类黄酮代谢。代谢组学结果表明， 的叶和根块中的类黄酮存在差异。共检测到 22 种类黄酮，叶中的大多数类黄酮浓度高于根块中的浓度。转录组分析表明，叶和根块中的差异表达基因（DEG）富集在光合作用天线蛋白中。Pearson 相关分析表明，查尔酮异构酶（CHI）和 UDP-葡萄糖类黄酮糖基转移酶（UFGT）的表达水平与大多数类黄酮的浓度高度相关。此外，本研究发现光合作用天线蛋白对 的类黄酮差异有重要贡献。中国浙江省杭州、金华、丽水和台州的叶片和根块的总类黄酮的基因表达和浓度表明，CHI（CL6715.Contig1_All、Unigene19431_All、CL921.Contig4_All）和 UFGT（CL11556.Contig3_All、CL11775.Contig1_All）是大多数类黄酮积累的潜在关键基因。
