• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一名患者两例根尖周炎病例的根管内微生物群特征：与唾液和牙菌斑特征的比较。

Intracanal microbiome profiles of two apical periodontitis cases in one patient: A comparison with saliva and plaque profiles.

作者信息

Yamaki Keiko, Tamahara Toru, Washio Jumpei, Sato Takuichi, Shimizu Ritsuko, Yamada Satoru

机构信息

Division of Periodontology and Endodontology, Graduate School of Dentistry, Tohoku University, Sendai, Japan.

Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan.

出版信息

Clin Exp Dent Res. 2024 Apr;10(2):e862. doi: 10.1002/cre2.862.

DOI:10.1002/cre2.862
PMID:38433294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10909803/
Abstract

OBJECTIVES

To determine the characteristics of the endodontic microbiome.

MATERIAL AND METHODS

Saliva, plaque, and infected root canal wall dentin of two teeth suffering from apical periodontitis were harvested from a 58-year-old man. Bacterial DNA was extracted from each sample, and 16S rRNA gene analysis targeting the V3-V4 region was conducted on the Illumina MiSeq platform using QIIME2. The functional potential of the microbiomes was inferred using PICRUSt2.

RESULTS

The four microbiomes were different in structure and membership, yet the nine most abundant metabolic pathways were common among them. The two endodontic microbiomes were more anaerobic, rich in Firmicutes, and scarce in Actinobacteriota and Proteobacteria, compared with saliva and plaque microbiomes. Their profiles were dissimilar despite their clinical and radiographic similarities.

CONCLUSIONS

The endodontic microbiomes were anaerobic, rich in Firmicutes, scarce in Actinobacteriota and Proteobacteria, and considerably varied within an individual.

摘要

目的

确定牙髓微生物群的特征。

材料与方法

从一名58岁男性身上采集了两颗患有根尖周炎牙齿的唾液、牙菌斑和感染根管壁牙本质。从每个样本中提取细菌DNA，并使用QIIME2在Illumina MiSeq平台上针对V3-V4区域进行16S rRNA基因分析。使用PICRUSt2推断微生物群的功能潜力。

结果

这四种微生物群在结构和组成上有所不同，但其中九个最丰富的代谢途径是共有的。与唾液和牙菌斑微生物群相比，这两个牙髓微生物群更厌氧，厚壁菌门丰富，放线菌门和变形菌门稀少。尽管它们在临床和影像学上相似，但其特征却不相同。

结论

牙髓微生物群是厌氧的，厚壁菌门丰富，放线菌门和变形菌门稀少，且个体内差异很大。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/17dce5bfcab8/CRE2-10-e862-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/4e5bcb5d0a3c/CRE2-10-e862-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/92084261ea60/CRE2-10-e862-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/c57f5c127045/CRE2-10-e862-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/44f82aa0628f/CRE2-10-e862-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/88d2184f58dd/CRE2-10-e862-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/aa8c1815e722/CRE2-10-e862-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/e5be3af9acfc/CRE2-10-e862-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/17dce5bfcab8/CRE2-10-e862-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/4e5bcb5d0a3c/CRE2-10-e862-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/92084261ea60/CRE2-10-e862-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/c57f5c127045/CRE2-10-e862-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/44f82aa0628f/CRE2-10-e862-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/88d2184f58dd/CRE2-10-e862-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/aa8c1815e722/CRE2-10-e862-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/e5be3af9acfc/CRE2-10-e862-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef0d/10909803/17dce5bfcab8/CRE2-10-e862-g005.jpg

相似文献

1
Intracanal microbiome profiles of two apical periodontitis cases in one patient: A comparison with saliva and plaque profiles.一名患者两例根尖周炎病例的根管内微生物群特征：与唾液和牙菌斑特征的比较。
Clin Exp Dent Res. 2024 Apr;10(2):e862. doi: 10.1002/cre2.862.
2
Root Canal Microbiome Associated With Asymptomatic Apical Periodontitis as Determined by High-Throughput Sequencing.高通量测序分析无症状性根尖周炎的根管微生物群。
J Endod. 2022 Apr;48(4):487-495. doi: 10.1016/j.joen.2022.01.012. Epub 2022 Jan 26.
3
Microbiota in the apical root canal system of tooth with apical periodontitis.根尖周炎患牙根管系统内的微生物群。
BMC Genomics. 2019 Apr 4;20(Suppl 2):189. doi: 10.1186/s12864-019-5474-y.
4
Metagenomic study of bacterial microbiota in persistent endodontic infections using Next-generation sequencing.使用下一代测序技术对持续性牙髓感染中细菌微生物群进行宏基因组学研究。
Int Endod J. 2018 Dec;51(12):1336-1348. doi: 10.1111/iej.12953. Epub 2018 Jun 9.
5
Host-microbiome interactions in apical periodontitis: The endodontic microbiome in relation to circulatory immunologic markers.根尖周炎中的宿主-微生物相互作用：牙髓微生物组与循环免疫标志物的关系。
Int Endod J. 2023 Jun;56(6):748-764. doi: 10.1111/iej.13912. Epub 2023 Apr 25.
6
Microbiome in paired root apices and periapical lesions and its association with clinical signs in persistent apical periodontitis using next-generation sequencing.使用新一代测序技术分析慢性根尖周炎患牙根尖与根尖周病变中的微生物群及其与临床症状的相关性
Int Endod J. 2023 May;56(5):622-636. doi: 10.1111/iej.13893. Epub 2023 Jan 30.
7
Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next-generation sequencing.基于下一代测序的根管填充牙临床症状与根管微生物组之间的潜在关系。
Int Endod J. 2022 Jan;55(1):18-29. doi: 10.1111/iej.13640. Epub 2021 Oct 28.
8
Highly diverse microbiota in dental root canals in cases of apical periodontitis (data of illumina sequencing).根尖周炎病例中根管内高度多样的微生物群（Illumina测序数据）
J Endod. 2014 Nov;40(11):1778-83. doi: 10.1016/j.joen.2014.06.017. Epub 2014 Sep 16.
9
Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis.与根尖周炎相关的唾液和已填充根管中粪肠球菌的流行率。
Int J Oral Sci. 2012 Mar;4(1):19-23. doi: 10.1038/ijos.2012.17. Epub 2012 Mar 16.
10
Impact of root canal preparation using two single-file systems on the intra-radicular microbiome of teeth with primary apical periodontitis.两种单文件系统根管预备对伴有原发性根尖周炎牙齿根管内微生物组的影响。
Clin Oral Investig. 2024 Feb 8;28(2):139. doi: 10.1007/s00784-024-05544-2.

引用本文的文献

1
Lactic Acid Bacteria in the Human Oral Cavity: Assessing Metabolic Functions Relevant to Oral Health and Disease.口腔中的乳酸菌：评估与口腔健康和疾病相关的代谢功能。
Methods Mol Biol. 2024;2851:151-172. doi: 10.1007/978-1-0716-4096-8_14.
2
Sequential Sampling of the Gastrointestinal Tract to Characterize the Entire Digestive Microbiome in Japanese Subjects.对日本受试者胃肠道进行序贯采样以表征整个消化微生物组
Microorganisms. 2024 Jun 28;12(7):1324. doi: 10.3390/microorganisms12071324.

本文引用的文献

1
Development of a Simple DNA Extraction Method and Pan Loop-Mediated Isothermal Amplification Assay for Diagnosis of Candidemia.一种用于念珠菌血症诊断的简单DNA提取方法及泛环介导等温扩增检测法的开发
Pathogens. 2022 Jan 18;11(2):111. doi: 10.3390/pathogens11020111.
2
Microbiome of Saliva and Plaque in Children According to Age and Dental Caries Experience.根据年龄和龋齿经历分析儿童唾液和牙菌斑的微生物群
Diagnostics (Basel). 2021 Jul 23;11(8):1324. doi: 10.3390/diagnostics11081324.
3
Patterns of Oral Microbiota in Patients with Apical Periodontitis.
根尖周炎患者口腔微生物群模式
J Clin Med. 2021 Jun 19;10(12):2707. doi: 10.3390/jcm10122707.
4
Influence of Bacterial Profiles in Cytokine and Clinical Features of Endodontic Disease.细菌谱对牙髓病细胞因子及临床特征的影响
J Endod. 2021 Aug;47(8):1265-1271. doi: 10.1016/j.joen.2021.04.026. Epub 2021 May 18.
5
Characterization of Root Canal Microbiota in Teeth Diagnosed with Irreversible Pulpitis.根管内牙髓病不可逆性牙髓炎的微生物群特征。
J Endod. 2021 Mar;47(3):415-423. doi: 10.1016/j.joen.2020.12.009. Epub 2020 Dec 23.
6
ppGpp functions as an alarmone in metazoa.ppGpp 在后生动物中作为警报素发挥作用。
Commun Biol. 2020 Nov 13;3(1):671. doi: 10.1038/s42003-020-01368-4.
7
The stringent response and physiological roles of (pp)pGpp in bacteria.(pp)pGpp 在细菌中的严格响应和生理作用。
Nat Rev Microbiol. 2021 Apr;19(4):256-271. doi: 10.1038/s41579-020-00470-y. Epub 2020 Nov 4.
8
PICRUSt2 for prediction of metagenome functions.用于宏基因组功能预测的PICRUSt2
Nat Biotechnol. 2020 Jun;38(6):685-688. doi: 10.1038/s41587-020-0548-6.
9
Profiling of Oral Bacterial Communities.口腔细菌群落分析。
J Dent Res. 2020 Jun;99(6):621-629. doi: 10.1177/0022034520914594. Epub 2020 Apr 14.
10
The MetaCyc database of metabolic pathways and enzymes - a 2019 update.代谢途径和酶的 MetaCyc 数据库——2019 年更新。
Nucleic Acids Res. 2020 Jan 8;48(D1):D445-D453. doi: 10.1093/nar/gkz862.