Romoli Ottavia, Henrion-Lacritick Annabelle, Blanc Hervé, Frangeul Lionel, Saleh Maria-Carla
Institut Pasteur, Université Paris Cité, CNRS UMR3569, Viruses and RNAi Unit, F-75015 Paris, France.
iScience. 2024 Feb 16;27(3):109261. doi: 10.1016/j.isci.2024.109261. eCollection 2024 Mar 15.
Mosquitoes, particularly , are critical vectors for globally significant pathogenic viruses. This study examines the limitations of oral RNA interference (RNAi) as a strategy to disrupt viral transmission by . We hypothesized that double-stranded RNA (dsRNA) targeting the Zika virus (ZIKV) or chikungunya virus (CHIKV) genomes produced by engineered bacterial symbionts could trigger an antiviral response. Mosquitoes mono-colonized with producing dsZIK or dsCHIK did not display reduced viral titers following exposure to virus-contaminated bloodmeals and failed to generate dsZIK- or dsCHIK-derived small interfering RNAs. To address potential limitations of bacterial dsRNA release, we explored dsRNA inoculation via feeding and injection. Although viral replication was impeded in mosquitoes injected with dsZIK or dsCHIK, no antiviral effect was observed in dsRNA-fed mosquitoes. These findings highlight complexities of implementing oral RNAi as an antiviral strategy in and warrant further exploration of local and systemic RNAi mechanisms.
蚊子，尤其是[具体种类未提及]，是全球重要致病病毒的关键传播媒介。本研究考察了口服RNA干扰（RNAi）作为一种破坏[具体蚊子种类]病毒传播策略的局限性。我们假设，由工程化细菌共生体产生的靶向寨卡病毒（ZIKV）或基孔肯雅病毒（CHIKV）基因组的双链RNA（dsRNA）可引发抗病毒反应。用产生dsZIK或dsCHIK的[具体细菌种类未提及]单定殖的蚊子在接触受病毒污染的血餐后，病毒滴度并未降低，且未能产生源自dsZIK或dsCHIK的小干扰RNA。为解决细菌dsRNA释放的潜在局限性，我们探索了通过喂食和注射接种dsRNA的方法。尽管注射dsZIK或dsCHIK的蚊子中病毒复制受到阻碍，但在喂食dsRNA的蚊子中未观察到抗病毒效果。这些发现凸显了在[具体蚊子种类]中实施口服RNAi作为抗病毒策略的复杂性，值得进一步探索局部和全身RNAi机制。
