Diversity of midgut microbiota in laboratory-colonized and field-collected (Diptera: Culicidae): A preliminary study.

is an important vector for many pathogens. Previous studies have revealed a role for midgut bacteria during pathogen infection in mosquitoes; however, studies of midgut bacteria are limited. We examined the diversity of midgut bacteria in female laboratory-colonized and field-collected . A total of 31 bacterial genera were identified representing 10 and 28 genera of laboratory-colonized and field-collected , respectively. The predominant bacterial genera in the laboratory-colonized were and , whereas the bacterial diversity in the field-collected exhibited a higher proportion of and as the dominant genera. However, only showed a significant difference between laboratory-colonized and field-collected . The midgut bacterial species were identified from 30 laboratory-colonized mosquitoes. A total of 16 bacterial species were identified and the predominant bacterial species was , followed by and Field mosquitoes were collected from the Sing Buri, Chumphon, and Yala Provinces of Thailand. The midgut bacterial species identified from the 10 collected from the Sing Buri Province included and was the only bacteria identified from this area. Midgut bacterial species were identified from 40 filed-collected from Chumphon Province. A total of 25 bacterial species were identified and the predominant species were and Only 15 bacterial species were identified from the mosquitoes collected from Chumphon Province. A total of 18 bacterial species were identified from 30 collected from Yala Province and the predominant species were and Only 12 bacterial species were found in mosquitoes collected from Yala Province. These findings indicate changes in the midgut bacteria population in from various locales, which may result from variability in the blood-meal source, diet, or habitat. A comprehensive survey of the midgut bacteria community prevalence in wild populations is critical for not only gaining a better understanding of the role of this bacterium in shaping the microbial community in but also for informing current and future mosquito and disease control programs.