Pharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, 12622, Giza, Egypt.
Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, 12622, Giza, Egypt.
Chem Biodivers. 2024 May;21(5):e202400245. doi: 10.1002/cbdv.202400245. Epub 2024 Apr 12.
Despite Aloe's traditional use, Aloe juvenna Brandham & S.Carter is poorly characterized. Other Aloes are known for their antidiabetic activity. This study describes the antidiabetic potentials and phytoconstituents of the A. juvenna leaves methanolic extract (AJME). Twenty-six phytoconstituents of AJME were described using HPLC/MS-MS. Lupeol and vitexin were isolated using column chromatography. The antidiabetic activity of AJME was investigated using an in vivo high-fat diet/streptozotocin-induced diabetic rat model and in vitro α-glucosidase and α-amylase inhibitory activity assays. AJME demonstrated its α-amylase inhibitory activity (IC=313±39.9 ppm) with no effect on α-glucosidase. In vivo, AJME dose-dependently improved hyperglycaemia in a high-fat diet/streptozotocin-induced diabetic rat model. Notably, the higher dose (1600 mg/kg) of AJME significantly downregulated serum interleukin-6, tumor necrosis factor-α, and matrix metalloproteinase-1 genes, suggesting its anti-inflammatory effect. These findings indicate AJME's potential as a significant antidiabetic agent through its α-amylase inhibition, hypoglycaemic, and anti-inflammatory properties.
尽管芦荟有传统的用途，但 Brandham 和 S.Carter 等人描述的少年芦荟特征较差。其他芦荟以其抗糖尿病活性而闻名。本研究描述了少年芦荟叶甲醇提取物 (AJME) 的抗糖尿病潜力和植物成分。使用 HPLC/MS-MS 描述了 AJME 的 26 种植物成分。使用柱层析分离出羽扇醇和荭草苷。使用体内高脂肪饮食/链脲佐菌素诱导的糖尿病大鼠模型和体外α-葡萄糖苷酶和α-淀粉酶抑制活性测定研究了 AJME 的抗糖尿病活性。AJME 表现出其对 α-淀粉酶的抑制活性（IC=313±39.9 ppm），对 α-葡萄糖苷酶没有影响。在体内，AJME 剂量依赖性地改善高脂肪饮食/链脲佐菌素诱导的糖尿病大鼠模型中的高血糖症。值得注意的是，AJME 的较高剂量（1600 mg/kg）显著下调了血清白细胞介素-6、肿瘤坏死因子-α 和基质金属蛋白酶-1 基因，表明其具有抗炎作用。这些发现表明 AJME 通过其对 α-淀粉酶的抑制、降血糖和抗炎特性，具有作为一种重要抗糖尿病药物的潜力。
