Beijing Municipal Research Institute of Eco-Environmental Protection, Beijing, 100037, China.
National Engineering Research Centre of Urban Environmental Pollution Control, Beijing, 100037, China.
Environ Geochem Health. 2024 Mar 4;46(3):103. doi: 10.1007/s10653-024-01913-8.
In this study, we focused on soil contaminated by polycyclic aromatic hydrocarbons (PAHs) at typical coking-polluted sites in Beijing, conducted research on enhanced PAH bioremediation and methods to evaluate remediation effects based on toxicity testing, and examined changes in pollutant concentrations during ozone preoxidation coupled with biodegradation in test soil samples. The toxicity of mixed PAHs in soil was directly evaluated using the Ames test, and the correlation between mixed PAH mutagenicity and benzo(a)pyrene (BaP) toxicity was investigated in an effort to establish a carcinogenic risk assessment model based on biological toxicity tests to evaluate remediation effects on PAH-contaminated soil. This study provides a theoretical and methodological foundation for evaluating the effect of bioremediation on PAH-contaminated soil at industrially contaminated sites. The results revealed that the removal rate of PAHs after 5 min of O preoxidation and 4 weeks of soil reaction with saponin surfactants and medium was 83.22%. The soil PAH extract obtained after remediation had a positive effect on the TA98 strain at a dose of 2000 μg·dish, and the carcinogenic risk based on the Ames toxicity test was 8.98 times greater than that calculated by conventional carcinogenic PAH toxicity parameters. The total carcinogenic risk of the remediated soil samples was approximately one order of magnitude less than that of the original soil samples.
在这项研究中，我们专注于北京典型焦化污染场地中被多环芳烃（PAHs）污染的土壤，研究了基于毒性测试的增强型 PAH 生物修复和修复效果评估方法，并考察了在臭氧预氧化耦合生物降解过程中污染物浓度在试验土壤样品中的变化。采用 Ames 试验直接评价土壤中混合 PAHs 的毒性，并研究混合 PAH 致突变性与苯并(a)芘（BaP）毒性的相关性，旨在建立基于生物毒性试验的致癌风险评估模型，用于评估受 PAHs 污染土壤的修复效果。该研究为评价工业污染场地 PAH 污染土壤的生物修复效果提供了理论和方法基础。结果表明，O 预氧化 5 分钟和用皂素表面活性剂和介质进行 4 周土壤反应后，PAHs 的去除率为 83.22%。修复后的土壤 PAH 提取物在 2000μg·盘剂量下对 TA98 菌株有正向作用，基于 Ames 毒性试验的致癌风险比传统致癌 PAH 毒性参数计算的致癌风险高 8.98 倍。修复土壤样品的总致癌风险比原始土壤样品约低一个数量级。
