Frendewey D, Dingermann T, Cooley L, Söll D
J Biol Chem. 1985 Jan 10;260(1):449-54.
Transfer RNA biosynthesis is a complex process which includes size trimming and nucleotide modification of an initial tRNA precursor. We have examined the temporal order and the nature of tRNA processing events in a Drosophila in vitro transcription/processing system using Drosophila tRNA genes as templates. RNA sequence analysis of processing products indicates that processing at both 5' and 3' ends occurs by endonucleolytic cleavage. The time course of processing of an initial tRNA precursor to mature tRNA reveals that trimming at the 5' end precedes 3' end maturation.
转运RNA生物合成是一个复杂的过程，包括对初始tRNA前体进行大小修剪和核苷酸修饰。我们利用果蝇tRNA基因作为模板，在果蝇体外转录/加工系统中研究了tRNA加工事件的时间顺序和性质。加工产物的RNA序列分析表明，5'端和3'端的加工都是通过内切核酸酶切割进行的。初始tRNA前体加工成成熟tRNA的时间进程表明，5'端的修剪先于3'端的成熟。
