Lu Jiaojiao, Xing Guimei, Zhang Yanqiu, Zhang Huihua, Wu Tianyu, Tian Zengzhi, Qu Lianwei
Institute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, China.
Liaoning Provincial Key Laboratory of Floriculture, Shenyang, Liaoning, China.
Front Plant Sci. 2024 Feb 19;15:1346255. doi: 10.3389/fpls.2024.1346255. eCollection 2024.
Currently, soil heavy metal contamination is a severe issue, particularly with Cd pollution. The metal tolerance protein (MTP) proteins, as plant divalent cation transporters, play a crucial role in the transport and tolerance of heavy metals in plants. This study conducted comprehensive identification and characterization of the gene family in the tulip. A total of 11 genes were identified and phylogenetically classified into three subfamilies. Conserved motif and gene structure analyses unveiled commonalities and variations among subfamily members. Expression profiling demonstrated several s were markedly upregulated under Cd exposure, including the . Heterologous expression in yeast validated that could ameliorate Cd sensitivity and enhance its tolerance. These results provide primary insights into the gene family in tulip. Phylogenetic relationships and functional analyses establish a framework for elucidating the transporters and molecular mechanisms governing Cd accumulation and distribution in tulip. Key s identified, exemplified by , may illuminate molecular breeding efforts aimed at developing Cd-tolerant cultivars for the remediation of soil Cd contamination.
目前,土壤重金属污染是一个严重问题,尤其是镉污染。金属耐受蛋白(MTP)作为植物二价阳离子转运蛋白,在植物重金属转运和耐受中起关键作用。本研究对郁金香中的该基因家族进行了全面鉴定和表征。共鉴定出11个基因,并在系统发育上分为三个亚家族。保守基序和基因结构分析揭示了亚家族成员之间的共性和差异。表达谱分析表明,在镉暴露下,有几个基因显著上调,包括……。在酵母中的异源表达证实……可以改善镉敏感性并增强其耐受性。这些结果为郁金香中的该基因家族提供了初步见解。系统发育关系和功能分析为阐明郁金香中控制镉积累和分布的转运蛋白及分子机制建立了框架。以……为例鉴定出的关键基因,可能为旨在培育耐镉品种以修复土壤镉污染的分子育种工作提供启示。