Pho Mai, Berrada Yasmin, Gunda Aachal, Stephens Andrew D
Biology Department, University of Massachusetts Amherst, Amherst Center, Massachusetts, United States.
Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst Center, Massachusetts, United States.
MicroPubl Biol. 2024 Feb 16;2024. doi: 10.17912/micropub.biology.001103. eCollection 2024.
Lamin intermediate filaments form a peripheral meshwork to support nuclear shape and function. Knockout of the LMNA gene that encodes for both lamin A and C results in an abnormally shaped nucleus. To determine the relative contribution of lamin A and C to nuclear shape, we measured nuclear blebbing and circular deviation in separate lamin A and lamin C knockdown and LMNA-/- stable cells. Lamin A knockdown increased nuclear blebbing while loss of lamin A, C, or both increased circular deviation. Overall, loss of lamin A, lamin C or both lamin A/C affect nuclear shape differentially.
核纤层中间丝形成一个外周网络以支持细胞核的形状和功能。编码核纤层蛋白A和C的LMNA基因敲除会导致细胞核形状异常。为了确定核纤层蛋白A和C对细胞核形状的相对贡献，我们在单独的核纤层蛋白A和核纤层蛋白C敲低以及LMNA基因敲除稳定细胞中测量了核出泡和圆形偏差。核纤层蛋白A敲低增加了核出泡，而核纤层蛋白A、C或两者的缺失增加了圆形偏差。总体而言，核纤层蛋白A、核纤层蛋白C或两者的缺失对细胞核形状的影响存在差异。
