P颗粒抗体KT3可识别PGL-1和PGL-3中的表位。
The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3.
作者信息
Danlasky Brennan M, Martinez Mario, Cassani Madeline, Bodas Devavrat, Putnam Andrea, Seydoux Geraldine
机构信息
Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine.
Molecular Biology and Genetics, University of Wisconsin-Madison, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine.
出版信息
MicroPubl Biol. 2024 Feb 16;2024. doi: 10.17912/micropub.biology.001137. eCollection 2024.
Abstract
The KT3 antibody is a commercially available antibody that recognizes the P granule protein PGL-3 (Takeda et al., 2008). Using immunostaining and western blotting of purified peptide fragments, we show that KT3 recognizes both PGL-3 and its paralog PGL-1 , likely through a shared epitope in the intrinsically disordered region.
摘要
KT3抗体是一种可商购的抗体，可识别P颗粒蛋白PGL-3（武田等人，2008年）。通过对纯化肽片段进行免疫染色和蛋白质印迹分析，我们发现KT3可能通过内在无序区域中的共享表位识别PGL-3及其旁系同源物PGL-1。
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