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赭曲霉毒素A的生物降解及培养变量的统计优化

Ochratoxin A biodegradation by and statistical optimization of cultural variables.

作者信息

Söylemez Tuncay, Yamaç Mustafa, Eninanç Ayşe Betül, Yıldız Zeki

机构信息

Institut Für Lebensmittelchemie, Gottfried Wilhelm Leibniz Universtät Hannover, Callinstraβe 5, 30167 Hannover, Germany.

Department of Biology, Faculty of Science and Letters, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey.

出版信息

Food Sci Biotechnol. 2023 Sep 14;33(5):1221-1231. doi: 10.1007/s10068-023-01417-8. eCollection 2024 Apr.

Abstract

The goal of this study is to identify the optimum conditions for ochratoxin A (OTA) biodegradation by the supernatant of strain. The Plackett-Burman and Box-Behnken methods were used to determine optimum OTA degradation conditions of under various incubation conditions. The Plackett-Burman method was planned through 16 varied experiments with 15 variants. The three most potent variants, sucrose, yeast extract and wheat bran, were selected using the Box-Behnken methodology. Ochratoxin A biodegradation ratio of 46.67% has been specified in only 1 h under ideal growing conditions. This is the first report on the optimization of OTA biodegradation by . When compared to previously published articles, it can be asserted that has promise based on its OTA biodegradation ratio in only 1 h of reaction time.

摘要

本研究的目的是确定菌株上清液对赭曲霉毒素A(OTA)进行生物降解的最佳条件。采用Plackett-Burman法和Box-Behnken法来确定在不同培养条件下OTA的最佳降解条件。Plackett-Burman法通过16个不同实验和15个变量进行规划。使用Box-Behnken方法选择了三种最有效的变量,即蔗糖、酵母提取物和麦麸。在理想生长条件下,仅1小时内OTA的生物降解率就达到了46.67%。这是关于优化OTA生物降解的首次报道。与之前发表的文章相比,可以断言,基于其在仅1小时反应时间内的OTA生物降解率,该菌株具有应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/217b/10908693/75cc729852d3/10068_2023_1417_Fig1_HTML.jpg

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