Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Mesih Deneshvari Hospital Shahid Beheshti Medical Sciences University, Tehran, Iran.
Iran Biomed J. 2024 Jan 1;28(1):53-8. doi: 10.61186/ibj.3845. Epub 2023 Oct 14.
MiR-34a and miR-126 mainly act as tumor suppressors and are often downregulated in various cancers, including non-small cell lung cancer (NSCLC). We aimed to determine the methylation status of miR-34a and miR-126 in NSCLC patients.
The current study included 63 paraffin-embedded NSCLC and paired adjacent normal tissues. After DNA extraction and bisulfite treatment, the methylation status of miR-34a and miR-126 were evaluated using the MSP method.
There was no statistically significant difference between tumor and normal tissues regarding the methylation status of miR-34a and miR-126 (p > 0.05). Moreover, we found no significant correlation between the methylation status of miR-34a and miR-126 with patients’ demographic parameters, including gender, age, and pathology subtype (p > 0.05).
Considering the low expression of mir-126 and mir-34 in NSCLC, more sensitive methods are recommended to be exploited for detecting the level of methylation or underlying mechanisms other than promoter hypermethylation in silencing these genes in NSCLC.
miR-34a 和 miR-126 主要作为肿瘤抑制因子，在包括非小细胞肺癌（NSCLC）在内的各种癌症中经常下调。我们旨在确定 NSCLC 患者中 miR-34a 和 miR-126 的甲基化状态。
本研究包括 63 例石蜡包埋的 NSCLC 及配对的相邻正常组织。提取 DNA 并进行亚硫酸氢盐处理后，采用 MSP 法评估 miR-34a 和 miR-126 的甲基化状态。
miR-34a 和 miR-126 的甲基化状态在肿瘤组织和正常组织之间无统计学差异（p>0.05）。此外，我们发现 miR-34a 和 miR-126 的甲基化状态与患者的人口统计学参数（包括性别、年龄和病理亚型）之间无显著相关性（p>0.05）。
鉴于 NSCLC 中 mir-126 和 mir-34 的低表达，建议采用更敏感的方法来检测这些基因在 NSCLC 中沉默的甲基化水平或除启动子高甲基化以外的潜在机制。
