State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, China.
Jiangxi Province Key Laboratory of Edible and Medicinal Resources Exploitation, Nanchang University, Nanchang, China.
Prep Biochem Biotechnol. 2024 Sep;54(8):1001-1016. doi: 10.1080/10826068.2024.2323196. Epub 2024 Mar 6.
With the industrialization and development of modern science, the application of enzymes as green and environmentally friendly biocatalysts in industry has been increased widely. Among them, lipase (EC. 3.1.1.3) is a very prominent biocatalyst, which has the ability to catalyze the hydrolysis and synthesis of ester compounds. Many lipases have been isolated from various sources, such as animals, plants and microorganisms, among which microbial lipase is the enzyme with the most diverse enzymatic properties and great industrial application potential. It therefore has promising applications in many industries, such as food and beverages, waste treatment, biofuels, leather, textiles, detergent formulations, ester synthesis, pharmaceuticals and medicine. Although many microbial lipases have been isolated and characterized, only some of them have been commercially exploited. In order to cope with the growing industrial demands and overcome these shortcomings to replace traditional chemical catalysts, the preparation of new lipases with thermal/acid-base stability, regioselectivity, organic solvent tolerance, high activity and yield, and reusability through excavation and modification has become a hot research topic.
随着工业化和现代科学的发展，酶作为绿色环保的生物催化剂在工业中的应用得到了广泛的增加。其中，脂肪酶（EC.3.1.1.3）是一种非常突出的生物催化剂，具有催化酯化合物水解和合成的能力。许多脂肪酶已从各种来源中分离出来，如动物、植物和微生物，其中微生物脂肪酶是酶特性最多样化、工业应用潜力巨大的酶。因此，它在许多行业具有广阔的应用前景，如食品和饮料、废物处理、生物燃料、皮革、纺织品、洗涤剂配方、酯合成、制药和医学。尽管已经分离和表征了许多微生物脂肪酶，但只有其中一些得到了商业开发。为了满足不断增长的工业需求，克服这些缺点，以替代传统的化学催化剂，通过挖掘和修饰来制备具有热/酸碱稳定性、区域选择性、有机溶剂耐受性、高活性和产率以及可重复使用性的新型脂肪酶已成为一个热门的研究课题。
