National Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.
Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
Anal Chem. 2024 Mar 19;96(11):4495-4504. doi: 10.1021/acs.analchem.3c05120. Epub 2024 Mar 6.
The molecular detection of multiple respiratory viruses provides evidence for the rational use of drugs and effective health management. Herein, we developed and tested the clinical performance of an electrohydrodynamic-driven nanobox-on-mirror platform (E-NoM) for the parallel, accurate, and sensitive detection of four respiratory viral antigens. The E-NoM platform uses gold-silver alloy nanoboxes as the core material with the deposition of a silver layer as a shell on the core surfaces to amplify and enable a reproducible Raman signal readout that facilitates accurate detection. Additionally, the E-NoM platform employs gold microelectrode arrays as the mirror with electrohydrodynamics to manipulate the fluid flow and enhance molecular interactions for an improved biosensing response. The presence of viral antigens binds the nanobox-based core-shell nanostructure on the gold microelectrode and creates the nanocavity with extremely strong "hot spots" to benefit sensitive analysis. Significantly, in a large clinical cohort with 227 patients, the designed E-NoM platform demonstrates the capability of screening respiratory infection with achieved clinical specificity, sensitivity, and accuracy of 100.0, 96.48, and 96.91%, respectively. It is anticipated that the E-NoM platform can find a position in clinical usage for respiratory disease diagnosis.
呼吸道多种病毒的分子检测为合理用药和有效健康管理提供了证据。在此，我们开发并测试了一种电动力学驱动的纳米盒-镜平台（E-NoM），用于平行、准确、灵敏地检测四种呼吸道病毒抗原。E-NoM 平台使用金银合金纳米盒作为核心材料，在核心表面沉积一层银作为外壳，以放大和实现可重复的拉曼信号读出，从而便于准确检测。此外，E-NoM 平台采用金微电极阵列作为镜子，利用电动力学来操纵流体流动，增强分子相互作用，以提高生物传感响应。病毒抗原的存在将基于纳米盒的核壳纳米结构结合到金微电极上，并形成具有极强“热点”的纳米腔，有利于灵敏分析。重要的是，在一个包含 227 名患者的大型临床队列中，设计的 E-NoM 平台表现出了筛查呼吸道感染的能力，实现了 100.0%、96.48%和 96.91%的临床特异性、灵敏度和准确率。预计 E-NoM 平台将在临床应用中找到呼吸道疾病诊断的位置。
