Department of Gastroenterology, General Hospital of Ningxia Medical University, Yinchuan, China.
Department of Anesthesiology, General Hospital of Ningxia Medical University, Yinchuan, China.
Cell Cycle. 2024 Feb;23(3):294-307. doi: 10.1080/15384101.2024.2324587. Epub 2024 Mar 6.
The increase of the level has been previously identified in various cancers including gastric cancer (GC), but how the exerts its carcinogenic role in GC remains unclear. Several studies revealed that contributes to cancer progression via its secretion of extracellular vehicles (EVs). Hence, it's designed to reveal the influence of -derived EVs (Fn-EVs) in GC progression. The tumor and adjacent tissues were collected from 30 GC patients, and the abundance of was found to be highly expressed in tumor samples. The ultracentrifugation was employed to isolate EVs from and (), which were labeled Fn-EVs and -EVs, respectively. After treating GC cells with Fn-EVs and -EVs, cell counting kit 8, colony formation, wound healing as well as transwell assay were performed, which revealed that Fn-EVs effectively enhanced oxaliplatin resistance, and facilitated cell proliferation, migration, invasion, and stemness in GC cells while -EVs exert no significant effect on GC cells. Besides, the stemness and DNA repair of GC cells were also enhanced by Fn-EVs, as revealed by the sphere-forming assay and the detection of stemness- and DNA repair-associated proteins by western blotting. analyses demonstrated that Fn-EVs administration not only promoted GC tumor growth and liver metastasis but also conferred GC tumor resistance to oxaliplatin resistance. This study first revealed the contributive role of in GC development via Fn-EVs, which provided a better perspective for manipulating in treating GC patients with malignant phenotypes.
水平的增加已在包括胃癌（GC）在内的各种癌症中被发现，但 如何在 GC 中发挥致癌作用仍不清楚。几项研究表明， 通过其细胞外囊泡（EVs）的分泌促进癌症进展。因此，本研究旨在揭示 -衍生的 EVs（Fn-EVs）在 GC 进展中的影响。从 30 名 GC 患者中收集肿瘤和相邻组织，发现肿瘤样本中 的丰度高度表达。采用超速离心法从 和 （）中分离 EVs，分别标记为 Fn-EVs 和 -EVs。用 Fn-EVs 和 -EVs 处理 GC 细胞后，进行细胞计数试剂盒 8 检测、集落形成、划痕愈合和 Transwell 检测，结果表明 Fn-EVs 可有效增强奥沙利铂耐药性，并促进 GC 细胞增殖、迁移、侵袭和干细胞特性，而 -EVs 对 GC 细胞无明显影响。此外，Fn-EVs 还增强了 GC 细胞的干细胞特性和 DNA 修复，通过球形成检测和 Western blot 检测干细胞和 DNA 修复相关蛋白得到证实。 分析表明，Fn-EVs 的给药不仅促进了 GC 肿瘤的生长和肝转移，而且赋予了 GC 肿瘤对奥沙利铂耐药性的耐药性。本研究首次通过 Fn-EVs 揭示了 在 GC 发展中的作用，为操纵 在治疗具有恶性表型的 GC 患者方面提供了更好的视角。
