Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an 271018, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an 271018, China.
Poult Sci. 2024 May;103(5):103592. doi: 10.1016/j.psj.2024.103592. Epub 2024 Feb 27.
Since September 2018, serious meningitis has been found on some breeding-duck farms in Shandong Province, China. A large number of ducks exhibit severe neurological symptoms. The ducks were randomly selected for laboratory testing. Duck brain samples were collected using standard sterile techniques, and the staphylococci isolates were detected in 404 (70.14%) out of 576 brain samples. A total of 525 coagulase-negative staphylococci (CoNS) strains were isolated, including 6 species: Staphylococcus sciuri (S. sciuri) (67.24%, 353/525), Staphylococcus epidermidis (S. epidermidis) (9.71%, 51/525), Staphylococcus saprophyticus (S. saprophyticus) (8.38%, 44/525), Staphylococcus lentus (S. lentus) (7.62%, 40/525), Staphylococcus haemolyticus (S. haemolyticus) (2.48%, 13/525), and Staphylococcus xylosus (S. xylosus) (4.57%, 24/525). Mixed strain infections were detected in 121 (29.95%) infected presentations. The antimicrobial susceptibility testing indicated that 40.38% of the isolates exhibited multi-drug resistance, and 53.90% of the strains were methicillin-resistant strains by amplification of the methicillin resistance gene (mecA) gene. Through experimental reproduction of the disease, we determined that the CoNS strains were the leading pathogens causing bacterial meningitis in ducks. Although these CoNS strains does not directly cause the death of sick ducks, they still cause large economic losses due to the retarded growth and development of the sick ducks, lower feed returns, and lower grades of processed duck products. The results of this study will contribute to our understanding of the epidemiology and pathogenesis of CoNS and be helpful in the prevention and treatment of the infection.
自 2018 年 9 月以来，中国山东省的一些养鸭场发现了严重的脑膜炎。大量鸭子表现出严重的神经症状。这些鸭子被随机挑选进行实验室检测。使用标准无菌技术采集鸭脑样本，在 576 个脑样本中检测到 404 个（70.14%）分离出葡萄球菌。共分离出 525 株凝固酶阴性葡萄球菌（CoNS），包括 6 个种：松鼠葡萄球菌（S. sciuri）（67.24%，353/525）、表皮葡萄球菌（S. epidermidis）（9.71%，51/525）、腐生葡萄球菌（S. saprophyticus）（8.38%，44/525）、迟缓葡萄球菌（S. lentus）（7.62%，40/525）、溶血葡萄球菌（S. haemolyticus）（2.48%，13/525）和木糖葡萄球菌（S. xylosus）（4.57%，24/525）。在 121 个（29.95%）感染病例中检测到混合菌株感染。药敏试验表明，40.38%的分离株表现出多药耐药性，通过扩增耐甲氧西林基因（mecA）基因，53.90%的菌株为耐甲氧西林菌株。通过疾病的实验再现，我们确定 CoNS 菌株是导致鸭细菌性脑膜炎的主要病原体。虽然这些 CoNS 菌株不会直接导致患病鸭子死亡，但由于患病鸭子的生长发育迟缓、饲料回报降低和加工鸭产品等级降低，它们仍会造成巨大的经济损失。本研究的结果将有助于我们了解 CoNS 的流行病学和发病机制，并有助于预防和治疗感染。
