Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt.
Int Microbiol. 2023 Nov;26(4):989-1000. doi: 10.1007/s10123-023-00354-0. Epub 2023 Apr 13.
The pathogenic potential of vancomycin and methicillin-resistant coagulase-negative Staphylococci (VMRCoNS) on Egyptian poultry farms has received little attention. Therefore, this study aims to study the prevalence of CoNS in imported poultry flocks and commercial poultry farms, evaluate the presence of virulence and antibiotic resistance genes (sea, seb, sec, sed, see, and mecA), and assess their pathogenicity in broiler chicks. Seven species were identified among 25 isolates, such as 8 S. gallinarum, 5 S. saprophyticus, 5 S. chromogens, 3 S. warneri, 2 S. hominis, 1 S. caprae, and 1 S. epidermidis. All isolates were resistant to clindamycin, doxycycline, vancomycin, methicillin, rifampicin, and penicillin. The mecA gene was confirmed in 14 isolates, while the sed gene was revealed in seven isolates. Commercial 1-day-old Ross broiler chicks were divided into eight groups of three replicates (10 birds/group): group Ӏ was negative control; groups (П, Ш, IV, V, VI, VII, and VIII) were subcutaneously inoculated with 10 CFUml of S. hominis, S. caprae, S. epidermidis, S. gallinarum, S. chromogens, S. warneri, and S. saprophyticus, respectively. Groups VIII and V had mortality rates of 100% and 20%, respectively, with no evidence of mortalities in the other groups. The highest re-isolation of CoNS species was recorded in groups VII, VIII, and V. Postmortem and histopathological examination revealed the common presence of polyserositis in the internal organs, and hepatic and myocardial necrosis in groups IV, V, and VI. These findings revealed the pathogenic potential of CoNS, so special attention must be directed toward their public health impact.
万古霉素和耐甲氧西林凝固酶阴性葡萄球菌（VMRCoNS）对埃及家禽养殖场的致病性潜力尚未得到充分关注。因此，本研究旨在研究进口家禽群和商业家禽养殖场中凝固酶阴性葡萄球菌的流行情况，评估其毒力和抗生素耐药基因（sea、seb、sec、sed、see 和 mecA）的存在情况，并评估其在肉鸡中的致病性。在 25 株分离株中鉴定出 7 个种，分别为 8 株鸡葡萄球菌、5 株腐生葡萄球菌、5 株变葡球菌、3 株华纳葡萄球菌、2 株人葡萄球菌、1 株山羊葡萄球菌和 1 株表皮葡萄球菌。所有分离株均对克林霉素、强力霉素、万古霉素、甲氧西林、利福平利福平和青霉素耐药。14 株分离株中证实存在 mecA 基因，7 株分离株中显示存在 sed 基因。将商业 1 日龄罗斯肉鸡分为 8 组，每组 3 个重复（每组 10 只）：第 Ӏ 组为阴性对照；第 (П, Ш, IV, V, VI, VII 和 VIII) 组分别通过皮下接种 10 CFUml 的人葡萄球菌、山羊葡萄球菌、表皮葡萄球菌、鸡葡萄球菌、变葡球菌、华纳葡萄球菌和腐生葡萄球菌。第 VIII 组和第 V 组的死亡率分别为 100%和 20%，其他组均无死亡。第 VII 组、第 VIII 组和第 V 组中 CoNS 种的再分离率最高。剖检和组织病理学检查显示，第 IV、V 和 VI 组的内部器官普遍存在多发性浆膜炎，第 IV、V 和 VI 组的肝和心肌坏死。这些发现表明 CoNS 具有致病性，因此必须特别关注其对公共卫生的影响。
