Lu Bowei, Lu Xu, Mu Manman, Meng Shuxian, Feng Yaqing, Zhang Yi
School of Chemical Engineering and Technology, Institute of Molecular Plus, Tianjin University, Tianjin, China.
Ministry of Health and Medical, General Hospital of Tianjin Medical University, Tianjin, China.
Heliyon. 2024 Feb 23;10(5):e26907. doi: 10.1016/j.heliyon.2024.e26907. eCollection 2024 Mar 15.
To meet the requirements of diagnosis and treatment, photodynamic therapy (PDT) is a promising cancer treatment with less side-effect. A series of novel BODIPY complexes (BODIPY-CDs) served as PDT agents were first reported to enhance the biocompatibility and water solubility of BODIPY matrix through the click reaction of alkynyl-containing BODIPY and azide-modified cyclodextrin (CD). BODIPY-CDs possessed superior water solubility due to the introduction of CD and their fluorescence emission apparently redshifted (>90 nm) on account of triazole units as the linkers compared to alkynyl-containing BODIPY. Moreover, all the BODIPY-CDs were no cytotoxicity toward NIH 3T3 in different drug concentrations from 12.5 to 200 μg/mL, and had a certain inhibitory effect on tumor HeLa cells. Particularly, BODIPY-β-CD exhibited high reactive oxygen species generation and excellent photodynamic therapy activity against HeLa cells compared to other complexes. The cell viability of BODIPY-β-CD was dramatically reduced up to 20% in the concentration of 100 μg/mL upon 808 nm laser irradiation. This architecture might provide a new opportunity to develop valuable photodynamic therapy agents for tumor cells.
为满足诊断和治疗的需求，光动力疗法（PDT）是一种前景广阔且副作用较小的癌症治疗方法。首次报道了一系列用作光动力疗法试剂的新型硼二吡咯配合物（BODIPY-CDs），通过含炔基的硼二吡咯与叠氮化物修饰的环糊精（CD）的点击反应，增强了硼二吡咯基质的生物相容性和水溶性。由于引入了环糊精，BODIPY-CDs具有优异的水溶性，并且与含炔基的硼二吡咯相比，由于作为连接体的三唑单元，它们的荧光发射明显红移（>90nm）。此外，所有的BODIPY-CDs在12.5至200μg/mL的不同药物浓度下对NIH 3T3均无细胞毒性，并且对肿瘤HeLa细胞具有一定的抑制作用。特别地，与其他配合物相比，BODIPY-β-CD对HeLa细胞表现出高活性氧生成和优异的光动力疗法活性。在808nm激光照射下，100μg/mL浓度的BODIPY-β-CD的细胞活力显著降低至20%。这种结构可能为开发用于肿瘤细胞的有价值的光动力疗法试剂提供新的机会。
