College of Ecology and Environment, Xinjiang University, Urumqi 830017, China; Key Laboratory of Oasis Ecology of Education Ministry, Urumqi 830017, China.
School of Environment Science and Engineering, Tongji University, Shanghai 200092, China.
Sci Total Environ. 2024 May 1;923:171497. doi: 10.1016/j.scitotenv.2024.171497. Epub 2024 Mar 6.
Lead (Pb) can disrupt plant gene expression, modify metabolite contents, and influence the growth of plants. Cuminum cyminum L. is highly adaptable to adversity, but molecular mechanism by which it responds to Pb stress is unknown. For this study, transcriptomic and metabolomic sequencing was performed on root tissues of C. cyminum under Pb stress. Our results showed that high Pb stress increased the activity of peroxidase (POD), the contents of malondialdehyde (MDA) and proline by 80.03 %, 174.46 % and 71.24 %, respectively. Meanwhile, Pb stress decreased the activities of superoxide dismutase (SOD) and catalase (CAT) as well as contents of soluble sugars and GSH, which thus affected the growth of C. cyminum. In addition, Pb stress influenced the accumulation and transport of Pb in C. cyminum. Metabolomic results showed that Pb stress affected eight metabolic pathways involving 108 differentially expressed metabolites, primarily amino acids, organic acids, and carbohydrates. The differentially expressed genes identified through transcriptome analysis were mainly involved the oxidation reductase activity, transmembrane transport, phytohormone signaling, and MAPK signaling pathway. The results of this study will help to understand the molecular mechanisms of C. cyminum response to Pb stress, and provide a basis for screening seeds with strong resistance to heavy metals.
铅（Pb）可以破坏植物基因表达、改变代谢物含量并影响植物生长。孜然芹对逆境具有高度适应性，但它响应 Pb 胁迫的分子机制尚不清楚。在这项研究中，对 Pb 胁迫下孜然芹根组织进行了转录组和代谢组测序。我们的结果表明，高 Pb 胁迫分别使过氧化物酶（POD）的活性、丙二醛（MDA）和脯氨酸的含量增加了 80.03%、174.46%和 71.24%。同时，Pb 胁迫降低了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性以及可溶性糖和 GSH 的含量，从而影响了孜然芹的生长。此外，Pb 胁迫影响了孜然芹中 Pb 的积累和运输。代谢组学结果表明，Pb 胁迫影响了涉及 108 个差异表达代谢物的 8 条代谢途径，主要涉及氨基酸、有机酸和碳水化合物。通过转录组分析鉴定的差异表达基因主要涉及氧化还原酶活性、跨膜运输、植物激素信号转导和 MAPK 信号转导途径。本研究的结果将有助于了解孜然芹对 Pb 胁迫的分子机制，并为筛选具有较强重金属抗性的种子提供依据。
