Bakshi Progati, Bhowmik Anindita, Ahsan Sunjukta, Alim Sharmin Rumi
Institute of Nutrition and Food Science University of Dhaka Dhaka Bangladesh.
Department of Food Engineering Bangabandhu Sheikh Mujibur Rahman Science and Technology University Gopalganj Bangladesh.
Food Sci Nutr. 2023 Dec 27;12(3):1995-2002. doi: 10.1002/fsn3.3896. eCollection 2024 Mar.
The presence of antibiotic-resistant pathogens in food is a serious public health concern nowadays. This study focuses on the isolation and characterization of potentially pathogenic and antimicrobial-resistant pathogens in chicken curry and potato smash samples collected from the canteens and cafeteria of Dhaka University in Bangladesh. Isolates were identified by their cultural, morphological, and biochemical tests (motility indole urease test, Kliger's iron agar test, catalase test, oxidase test, methyl red and Voges-Proskauer tests). The antibiotic susceptibility test was done by the disk diffusion method. The range of total bacterial count in the potato smash and chicken curry samples was from 1.4 × 10 to 1.6 × 10 CFU/g and from 2.4 × 10 to 2.6 × 10 CFU/g, respectively. , , , , , , , , , , , , and strains were isolated in both samples. Isolates were highly resistant to ampicillin (90.90%) followed by colistin (52.27%), azithromycin (27.27%), and tetracycline 25%. species had the highest rate of multiple antibiotic resistance (MAR; 62.5%), followed by species (50%). The isolated strains were further analyzed through PCR assay to detect virulent genes (EPEC: 229 bp, 450 bp, ETEC 322 bp, EHEC 534 bp, and EIEC 320 bp). One isolate had the A target gene under EPEC pathotypes. , as a fecal indicator, may indicate fecal contamination or poor and unhygienic food handling. The findings recommend further investigations to identify potential mechanisms of contamination and preventive measures to improve the food safety level in the canteens and restaurants.
如今，食品中存在抗生素抗性病原体是一个严重的公共卫生问题。本研究聚焦于从孟加拉国达卡大学食堂和餐厅收集的咖喱鸡和土豆泥样本中潜在致病和抗微生物病原体的分离与特性分析。通过培养、形态学和生化试验（动力吲哚尿素酶试验、克氏铁琼脂试验、过氧化氢酶试验、氧化酶试验、甲基红和Voges - Proskauer试验）对分离株进行鉴定。采用纸片扩散法进行抗生素敏感性试验。土豆泥和咖喱鸡样本中的总细菌计数范围分别为1.4×10至1.6×10 CFU/g和2.4×10至2.6×10 CFU/g。在两个样本中均分离出了 、 、 、 、 、 、 、 、 、 、 、 和 菌株。分离株对氨苄西林的耐药性最高（90.90%），其次是黏菌素（52.27%）、阿奇霉素（27.27%）和四环素（25%）。 菌属的多重抗生素耐药率（MAR）最高（62.5%），其次是 菌属（50%）。对分离出的 菌株进一步通过PCR检测分析毒力基因（肠致病性大肠杆菌：229 bp， 450 bp，肠产毒性大肠杆菌322 bp，肠出血性大肠杆菌534 bp，和肠侵袭性大肠杆菌320 bp）。一株 分离株在肠致病性大肠杆菌致病型下有A靶基因。 作为粪便指示菌，可能表明粪便污染或食品处理不当且不卫生。研究结果建议进一步开展调查，以确定潜在的污染机制和预防措施，从而提高食堂和餐厅的食品安全水平。
